antibody

Goat anti-FOXA1/HNF3A Antibody

MBS420148

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, cow

western blot, immunohistochemistry, enzyme immunoassay, chromatin immunoprecipitation

Antibody

Goat anti-FOXA1/HNF3A Antibody

FOXA1/HNF3A

FOXA1; HNF3A; forkhead box A1; TCF3A; MGC33105; hepatocyte nuclear factor 3, alpha; FOXA1 antibody; HNF3A antibody; forkhead box A1 antibody; TCF3A antibody; MGC33105 antibody; hepatocyte nuclear factor 3; alpha antibody; FOXA1 / HNF3A

hepatocyte nuclear factor 3-alpha; Hepatocyte nuclear factor 3-alpha; hepatocyte nuclear factor 3-alpha; forkhead box A1; Forkhead box protein A1; Transcription factor 3A; TCF-3A

FOXA1

HNF3A

FOXA1; FOXA1; HNF3A; TCF3A; HNF3A; TCF3A; HNF-3-alpha; HNF-3A; TCF-3A

FOXA1_HUMAN

Polyclonal

Goat

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

472

GVYSRPVLNTS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Prostate shows preferential nuclear staining in the epithelial cells. Recommended concentration, 2-4ug/ml. Western Blot: Approx 50kDa band observed in lysates of cell line HepG2 (calculated MW of 49.1kDa according to NP_004487.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Chromatin Immunoprecipitation: This antibody has been successfully used for mouse embryonic stem cells and it has been confirmed in ChIP by anonymous customers.

Immunogen: Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2. Epitope: C Terminus

24497501

NP_004487.2

NM_004496.3

45,468 Da

Direct P53 Effectors Pathway (137939); FOXA Transcription Factor Networks Pathway (138065); FOXA1 Transcription Factor Network Pathway (137979); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Prostate Cancer Pathway (755440)

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]

FOXA1: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles. Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of p27Kip1, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis- acting regulatory regions of these genes. Involved in glucose homeostasis. Binds DNA as a monomer. Interacts with FOXA2. Interacts with NKX2-1. Interacts with HDAC7. Interacts with the histone H3-H4 heterodimer. Associates with nucleosomes containing histone H2A. Interacts with AR. Interacts with NR0B2. Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 14q21.1. Cellular Component: microvillus; nucleolus; nucleus. Molecular Function: DNA binding; protein domain specific binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding. Biological Process: anatomical structure formation; chromatin remodeling; dorsoventral neural tube patterning; glucose homeostasis; hormone metabolic process; negative regulation of transcription from RNA polymerase II promoter; neuron fate specification; Notch signaling pathway; positive regulation of estrogen receptor signaling pathway; positive regulation of mitotic cell cycle; positive regulation of neuron differentiation; positive regulation of smoothened signaling pathway; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to estradiol stimulus; transcription from RNA polymerase II promoter

0.1 mg

300 USD