catalog number :
MBS420146
products full name :
Goat anti-Choline acetyltransferase Antibody
products short name :
Choline acetyltransferase
products name syn :
choline acetyltransferase; CHAT; CMS1A; CMS1A2; acetyl CoA:choline O-acetyltransferase; CHAT; choline acetyltransferase; CMS1A; CMS1A2; acetyl CoA:choline O-acetyltransferase
other names :
choline O-acetyltransferase isoform 1; Choline O-acetyltransferase; choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase; choline O-acetyltransferase
products gene name :
CHAT
other gene names :
CHAT; CHAT; CMS1A; CMS1A2; CHOACTASE; CHOACTase; ChAT; Choline acetylase
uniprot entry name :
CLAT_HUMAN
reactivity :
Human, Dog, Pig, Cow
sequence :
CKEKATRPSQGHQP
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 70.5kDa according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence CKEKATRPSQGHQP, from the C Terminus of the protein sequence according to NP_066264.3; NP_065574.3; NP_001136405.1. Corresponding Immunizing Peptide: Catalog #MBS425778
other info2 :
Important Note: This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1). Reported variants represent identical protein (NP_066264.3; NP_066265.3; NP_066266.3; NP_001136406.1; NP_001136401.1).
ncbi acc num :
NP_066264.3
ncbi gb acc num :
NM_020984.3
ncbi mol weight :
70,394 Da
ncbi pathways :
Acetylcholine Neurotransmitter Release Cycle Pathway 106525!!Acetylcholine Synthesis Pathway 198820!!Biogenic Amine Synthesis Pathway 198793!!Cholinergic Synapse Pathway 217716!!Glycerophospholipid Biosynthesis Pathway 645313!!Glycerophospholipid Metabolism Pathway 82989!!Glycerophospholipid Metabolism Pathway 364!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!Neuronal System Pathway 106513
ncbi summary :
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]