catalog number :
MBS420133
products full name :
Goat anti-HNF4A Antibody
products short name :
HNF4A
products name syn :
HNF4A; TCF; HNF4; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; hepatocyte nuclear factor 4; alpha; HNF4-alpha; transcription factor-14; FLJ39654; HNF4alpha; MODY; MODY1; HNF4alpha10/11/12; HNF4A; TCF; HNF4; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; hepatocyte nuclear factor 4, alpha; HNF4-alpha; transcription factor-14; FLJ39654; HNF4alpha; MODY; MODY1; HNF4alpha10/11/12; hepatic nuclear factor 4 alpha
other names :
hepatocyte nuclear factor 4-alpha isoform HNF4alpha1; Hepatocyte nuclear factor 4-alpha; hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1; hepatocyte nuclear factor 4, alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4
products gene name :
HNF4A
other gene names :
HNF4A; HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha; HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14
uniprot entry name :
HNF4A_HUMAN
reactivity :
Human, Dog, Pig
sequence :
RLSKTLVDMDMADY
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 52kDa and 48kDa bands observed in lysates of Human hepatocellular carcinoma cell line HepG2 (calculated MW of 52.8kDa according to NP_000448.3 and 46.6kDa according to NP_849181.1). Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: N Terminus. Immunogen: Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1; NP_000448.3; NP_849181.1. Corresponding Immunizing Peptide: Catalog #MBS426029
other info2 :
Important Note: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
ncbi acc num :
NP_849180.1
ncbi gb acc num :
NM_178849.2
ncbi mol weight :
43,988 Da
ncbi pathways :
AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Developmental Biology Pathway 477129!!FOXA2 And FOXA3 Transcription Factor Networks Pathway 137911!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!HIF-1-alpha Transcription Factor Network Pathway 138045!!Integrated Pancreatic Cancer Pathway 711360!!Maturity Onset Diabetes Of The Young Pathway 83096
ncbi summary :
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]