antibody

Goat anti-CYP26B1 Antibody

MBS420112

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-CYP26B1 Antibody

CYP26B1

CYP26B1; cytochrome P450, family 26, subfamily B, polypeptide 1; CYP26A2; DKFZp686G0638; MGC129613; P450RAI-2; cytochrome P450 retinoid metabolizing protein; cytochrome P450, family 26, subfamily b, polypeptide 1; retinoic acid-metabolizing cytochrome; CYP26B1 antibody; cytochrome P450; family 26; subfamily B; polypeptide 1 antibody; CYP26A2 antibody; DKFZp686G0638 antibody; MGC129613 antibody; P450RAI-2 antibody; cytochrome P450 retinoid metabolizing protein antibody; cytochrome P450; family 26; subfamily b; polypeptide 1 antibody; retinoic acid-metabolizing cytochrome antibody

cytochrome P450 26B1 isoform 1; Cytochrome P450 26B1; cytochrome P450 26B1; cytochrome P450 family 26 subfamily B member 1; Cytochrome P450 26A2; Cytochrome P450 retinoic acid-inactivating 2; Cytochrome P450RAI-2; Retinoic acid-metabolizing cytochrome

CYP26B1

CYP26B1; CYP26B1; RHFCA; CYP26A2; P450RAI2; P450RAI-2; CYP26A2; P450RAI2; Cytochrome P450RAI-2

CP26B_HUMAN

Polyclonal

Goat

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog

512

SQARSEDKDGRFH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of cell bodies in the Molecular Layer. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave an approx 85kDa band in human testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_063938.1. The 85kDa band was successfully blocked by incubation with the immunizing peptide.

Immunogen: Peptide with sequence C-SQARSEDKDGRFH, from the internal region of the protein sequence according to NP_063938.1. Epitope: Internal region

9845285

NP_063938.1

NM_019885.3

55,756 Da

Adipogenesis Pathway (198832); Biological Oxidations Pathway (1270189); Cytochrome P450 - Arranged By Substrate Type Pathway (1270191); Metabolic Pathways (132956); Metabolism Pathway (1269956); Phase 1 - Functionalization Of Compounds Pathway (1270190); RA Biosynthesis Pathway (1269631); Retinol Metabolism Pathway (83020); Retinol Metabolism Pathway (406); Signal Transduction Pathway (1269379)

This gene encodes a member of the cytochrome P450 superfamily. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein is localized to the endoplasmic reticulum, and functions as a critical regulator of all-trans retinoic acid levels by the specific inactivation of all-trans retinoic acid to hydroxylated forms. Mutations in this gene are associated with radiohumeral fusions and other skeletal and craniofacial anomalies, and increased levels of the encoded protein are associated with atherosclerotic lesions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

CYP26B1: Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA 9-cis-RA 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH- RA. Esential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints. Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA). A disease characterized by craniofacial malformations, occipital encephalocele, radiohumeral fusions, oligodactyly, advanced osseous maturation, and calvarial mineralization defects. Belongs to the cytochrome P450 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Cell development/differentiation; EC 1.14.-.-. Chromosomal Location of Human Ortholog: 2p13.2. Cellular Component: cytoplasm; endoplasmic reticulum membrane. Molecular Function: heme binding; iron ion binding; retinoic acid 4-hydroxylase activity; retinoic acid binding. Biological Process: cell fate determination; embryonic limb morphogenesis; establishment of T cell polarity; inflammatory response; male meiosis; negative regulation of retinoic acid receptor signaling pathway; proximal/distal pattern formation; regulation of T cell differentiation; retinoic acid receptor signaling pathway; spermatogenesis; tongue morphogenesis; vitamin metabolic process. Disease: Radiohumeral Fusions With Other Skeletal And Craniofacial Anomalies

0.1 mg

300 USD