antibody

Goat anti-Insulysin / Insulinase Antibody

MBS420078

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, dog

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-Insulysin / Insulinase Antibody

Insulysin/Insulinase

insulinase; insulysin; insulin-degrading enzyme; FLJ35968; INSULYSIN; IDE; insulinase; insulysin; insulin-degrading enzyme; FLJ35968; INSULYSIN

insulysin; Insulin-degrading enzyme; insulin-degrading enzyme; insulinase; insulin protease; Abeta-degrading protease; insulin-degrading enzyme; Abeta-degrading protease; Insulin protease; Insulinase; Insulysin

IDE

IDE; IDE; INSULYSIN; Insulinase

IDE_HUMAN

Polyclonal

Goat

Human, Dog

1019

QQYNFDRDNTE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry: In paraffin embedded Human Lung shows cytoplasm staining of alveolar macrophages. Recommended concentration, 3-6ug/ml. Western Blot: Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). In transfected HEK293 transiently expressing Insulysin a band of approx. 110kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.

Epitope: Internal Region. Immunogen: Peptide with sequence C-QQYNFDRDNTE, from the internal region of the protein sequence according to NP_004960.1, NP_001159418.1. Corresponding Immunizing Peptide: Catalog #MBS425175

Important Note: This antibody is expected to recognize both reported isoforms (NP_004960.1 and NP_001159418.1).

4826770

NP_004960.1,

NM_004969.1

54,240 Da

Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

0.1 mg

290 USD