Goat anti-RNF213/C17orf27 (C Terminus) Antibody | MyBioSource MBS420069 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-RNF213/C17orf27 (C Terminus) Antibody

catalog :

MBS420069

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420069

products type :

Antibody

products full name :

Goat anti-RNF213/C17orf27 (C Terminus) Antibody

products short name :

RNF213/C17orf27

products name syn :

Goat Anti-RNF213 / C17orf27 (internal) Antibody; RNF213; C17orf27; ring finger protein 213; chromosome 17 open reading frame 27; KIAA1554; RNF213 antibody; C17orf27 antibody; ring finger protein 213 antibody; chromosome 17 open reading frame 27 antibody; KIAA1554 antibody; RNF213 / C17orf27 (C Terminus)

other names :

E3 ubiquitin-protein ligase RNF213; E3 ubiquitin-protein ligase RNF213; ring finger protein 213; ALK lymphoma oligomerization partner on chromosome 17

products gene name :

RNF213

products gene name syn :

C17orf27

other gene names :

RNF213; RNF213; ALO17; MYMY2; MYSTR; NET57; C17orf27; KIAA1618

uniprot entry name :

RN213_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human

sequence length :

557

sequence :

KTAAVLKWNRE

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded human kidney shows rare staining of nucleoli across the kidney. Recommended concentration, 2.5ug/ml. Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration and the product is investigative grade.

other info1 :

Immunogen: Peptide with sequence C-KTAAVLKWNRE, from the C Terminus of the protein sequence according to NP_065965.4. Epitope: C Terminus

ncbi gi num :

262231746

ncbi acc num :

NP_065965.4

ncbi gb acc num :

NM_020914.4

ncbi mol weight :

64,260 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1269193); Class I MHC Mediated Antigen Processing Presentation Pathway (1269192); Immune System Pathway (1269170)

ncbi summary :

This gene encodes a protein containing a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions. The protein also contains an AAA domain, which is associated with ATPase activity. This gene is a susceptibility gene for Moyamoya disease, a vascular disorder of intracranial arteries. This gene is also a translocation partner in anaplastic large cell lymphoma and inflammatory myofibroblastic tumor cases, where a t(2;17)(p23;q25) translocation has been identified with the anaplastic lymphoma kinase (ALK) gene on chromosome 2, and a t(8;17)(q24;q25) translocation has been identified with the MYC gene on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

uniprot summary :

RNF213: Probable E3 ubiquitin-protein ligase that may play a role in angiogenesis. May also have an ATPase activity. Defects in RNF213 are the cause of susceptibility to Moyamoya disease type 2 (MYMY2). A progressive cerebral angiopathy characterized by bilateral intracranial carotid artery stenosis and telangiectatic vessels in the region of the basal ganglia. The abnormal vessels resemble a 'puff of smoke' (moyamoya) on cerebral angiogram. Affected individuals can develop transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage. Hemiplegia of sudden onset and epileptic seizures constitute the prevailing presentation in childhood, while subarachnoid bleeding occurs more frequently in adults. A chromosomal aberration involving ALO17 is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALK. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.3.2.-; Oncoprotein; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 17q25.3. Cellular Component: cytoplasm; cytosol; membrane; nucleolus. Molecular Function: ATPase activity; ligase activity; protein binding; ubiquitin-protein ligase activity; zinc ion binding. Biological Process: angiogenesis; protein autoubiquitination; protein homooligomerization; protein polyubiquitination; protein ubiquitination; sprouting angiogenesis; ubiquitin-dependent protein catabolic process

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage