catalog number :
MBS420057
products full name :
Goat anti-OGT Antibody
products short name :
OGT
products name syn :
OGT; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); FLJ23071; HRNT1; MGC22921; O-GLCNAC; O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase; OGT; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); FLJ23071; HRNT1; MGC22921; O-GLCNAC; O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
other names :
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 1; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine (GlcNAc) transferase; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT
other gene names :
OGT; OGT; HRNT1; O-GLCNAC; OGT
uniprot entry name :
OGT1_HUMAN
reactivity :
Human, Mouse, Rat, Dog
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 110kDa band observed in Pancreas lysates from Rat and Mouse (calculated MW of 117kDa according to NP_858058.1. Recommended concentration: 0.05-0.2ug/ml.
An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. Peptide ELISA: antibody detection limit dilution 1:64000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-YEHPKDLKLSDGR, from the internal region of the protein sequence according to NP_858058.1; NP_858059.1. Corresponding Immunizing Peptide: Catalog #MBS426858
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1
ncbi acc num :
NP_858058.1
ncbi gb acc num :
NM_181672.2
ncbi mol weight :
74,536 Da
ncbi pathways :
Chromatin Modifying Enzymes Pathway 941403!!Chromatin Organization Pathway 941402!!HATs Acetylate Histones Pathway 941404!!Other Types Of O-glycan Biosynthesis Pathway 82978!!Other Types Of O-glycan Biosynthesis Pathway 349!!Protein O-[N-acetyl]-glucosylation Pathway 982231
ncbi summary :
This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]