catalog number :
MBS420018
products full name :
Goat anti-FZD8 / frizzled 8 Antibody
products short name :
FZD8/frizzled 8
products name syn :
FZD8; frizzled homolog 8 (Drosophila); HGNC:4046; FZ-8; hFZ8; frizzled (Drosophila) homolog 8; frizzled 8; FZD8; frizzled homolog 8 (Drosophila); HGNC:4046; FZ-8; hFZ8; frizzled (Drosophila) homolog 8; frizzled 8
other names :
frizzled-8; Frizzled-8; frizzled-8; frizzled homolog 8; frizzled family receptor 8; frizzled 8, seven transmembrane spanning receptor; frizzled class receptor 8
products gene name :
FZD8
other gene names :
FZD8; FZD8; FZ-8; hFZ8; Fz-8; hFz8
uniprot entry name :
FZD8_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Immunohistochemistry: In paraffin embedded Human Pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3ug/ml. Western Blot: Approx 75kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425467
ncbi acc num :
NP_114072.1
ncbi gb acc num :
NM_031866.2
ncbi mol weight :
73,300 Da
ncbi pathways :
Asymmetric Localization Of PCP Proteins Pathway 941396!!Basal Cell Carcinoma Pathway 83113!!Basal Cell Carcinoma Pathway 525!!Class B/2 (Secretin Family Receptors) Pathway 106378!!Disease Pathway 530764!!GPCR Ligand Binding Pathway 161020!!HTLV-I Infection Pathway 373901!!HTLV-I Infection Pathway 373889!!Hippo Signaling Pathway 749777!!Hippo Signaling Pathway 750388
ncbi summary :
This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008]