Goat anti-DGAT2 Antibody | MyBioSource MBS420006 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-DGAT2 Antibody

catalog :

MBS420006

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420006

products type :

Antibody

products full name :

Goat anti-DGAT2 Antibody

products short name :

DGAT2

products name syn :

DGAT2; diacylglycerol O-acyltransferase homolog 2 (mouse); DKFZp686A15125; HMFN1045; GS1999full; diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2 isoform 1; DGAT2 antibody; diacylglycerol O-acyltransferase homolog 2 (mouse) antibody; DKFZp686A15125 antibody; HMFN1045 antibody; GS1999full antibody; diacylglycerol O-acyltransferase homolog 2 antibody; diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody

other names :

diacylglycerol O-acyltransferase 2 isoform 1; Diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76); ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase 2

products gene name :

DGAT2

other gene names :

DGAT2; DGAT2; ARAT; HMFN1045; GS1999FULL; ARAT; Retinol O-fatty-acyltransferase

uniprot entry name :

DGAT2_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

sequence length :

388

sequence :

KLEHPTQQDIDLYH

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), ELISA (EIA)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3ug/ml. Western Blot: In transfected HEK293 transiently expressing DGAT2 bands of approx. 60kDa and 45kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in Human Liver lysates. The calculated molecular size is 43.8kDa according to NP_115953.2. Recommended concentration, 0.3-1ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant DGAT2 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml.

other info1 :

Immunogen: Peptide with sequence C-KLEHPTQQDIDLYH, from the internal region (near the C Terminus) of the protein sequence according to NP_115953.2. Epitope: Internal region (near the C Terminus)

other info2 :

Note: This antibody is not expected to cross-react with other DGAT members.

ncbi gi num :

26024197

ncbi acc num :

NP_115953.2

ncbi gb acc num :

NM_032564.4

ncbi mol weight :

39,044 Da

ncbi pathways :

Acyl Chain Remodeling Of DAG And TAG Pathway (1270056); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (1270010); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Glycerophospholipid Biosynthesis Pathway (1270054); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001)

ncbi summary :

This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

DGAT2: Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT). Belongs to the diacylglycerol acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; EC 2.3.1.20; Transferase; EC 2.3.1.76; Lipid Metabolism - glycerolipid; Cofactor and Vitamin Metabolism - retinol; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q13.5. Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to membrane; lipid particle; mitochondrion; perinuclear region of cytoplasm. Molecular Function: 2-acylglycerol O-acyltransferase activity; diacylglycerol O-acyltransferase activity; protein homodimerization activity; retinol O-fatty-acyltransferase activity. Biological Process: cholesterol homeostasis; diacylglycerol metabolic process; fatty acid homeostasis; glycerol metabolic process; negative regulation of fatty acid oxidation; positive regulation of gluconeogenesis; regulation of lipoprotein metabolic process; retinol metabolic process; sequestering of lipid; triacylglycerol biosynthetic process

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage