catalog number :
MBS420001
products full name :
Goat anti-4E-T / EIF4ENIF1 Antibody
products short name :
4E-T/EIF4ENIF1
products name syn :
EIF4ENIF1; 4E-T; Clast4; FLJ21601; 2610509L04Rik; eukaryotic translation initiation factor 4E nuclear import factor 1; eIF4E-transporter; FLJ26551; OTTHUMP00000063276; EIF4ENIF1; 4E-T; Clast4; FLJ21601; 2610509L04Rik; eukaryotic translation initiation factor 4E nuclear import factor 1; eIF4E-transporter; FLJ26551; OTTHUMP00000063276
other names :
eukaryotic translation initiation factor 4E transporter isoform a; Eukaryotic translation initiation factor 4E transporter; eukaryotic translation initiation factor 4E transporter; 2610509L04Rik; eIF4E transporter; eIF4E-transporter; eukaryotic translation initiation factor 4E nuclear import factor 1; Eukaryotic translation initiation factor 4E nuclear import factor 1
products gene name :
EIF4ENIF1
other gene names :
EIF4ENIF1; EIF4ENIF1; 4E-T; Clast4; 4E-T; eIF4E transporter
uniprot entry name :
4ET_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Pig, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10ug/ml. Western Blot: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below)). Recommended for use at 0.25-0.5ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-AKVISVDELEYRQ, from the C Terminus of the protein sequence according to NP_062817.2; NP_001157974.1. Corresponding Immunizing Peptide: Catalog #MBS426008
other info2 :
Important Note: This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
ncbi acc num :
NP_062817.2
ncbi gb acc num :
NM_019843.3
ncbi mol weight :
108,329 Da
ncbi summary :
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]