antibody

Anti-Histone H3.1 (Ab-10) Antibody

MBS417574

0.1 mg

405 USD

polyclonal

rabbit

nonconjugated

human, mouse

Antibody

Anti-Histone H3.1 (Ab-10) Antibody

[Histone H3-1]

[histone H3.1; Histone H3.1; histone H3.1; H3 histone family, member C; histone 1, H3c; histone H3/c; histone cluster 1, H3c; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l]

[HIST1H3C; HIST1H3A; H3.1; H3/c; H3FC]

H31_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

136

Histone H3.1 (Ab-10) antibody detects endogenous levels of total Histone H3.1 protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid

1ug/ul

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

WB: 1:500~1:1000

Test Data

Storage Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibody Type: Primary antibody

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-SP-T-G).

Dai J, et al. (2005) Genes Dev 19(4): 472-488. Yih LH, et al. (2005) Carcinogenesis 26(1): 53-63.

4504285

NP_003522.1

NM_003531.2

15,404 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (366238); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Condensation Of Prophase Chromosomes Pathway (1127547)

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Jul 2008]

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p22.2. Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; DNA replication-dependent nucleosome assembly; regulation of gene expression, epigenetic; DNA methylation on cytosine

0.1 mg

405 USD