antibody

Anti-PPP2R1A Antibody

MBS417083

0.1 mg

230 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Anti-PPP2R1A Antibody

[PPP2R1A]

[Homo sapiens protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, mRNA; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; medium tumor antigen-associated 61 KDA protein; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform; protein phosphatase 2, regulatory subunit A, alpha; Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha]

[PPP2R1A]

[PPP2R1A; PPP2R1A; PR65A; PP2AAALPHA; PP2A-Aalpha]

2AAA_HUMAN

Polyclonal

Rabbit

Human. Predicted: Rat, Mouse. Not yet tested in other species

2282

Affinity purified

Liquid

100ug/100ul

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

ELISA titer: 1:20,000-1:80,000. Western blot analysis: 1:200-1:1,000. Immunohistochemistry: 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user

Western Blot

Immunohistochemistry

Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.

Immunogen: Rabbit polyclonal PPP2R1A (1) antibody was raised against a recombinate human PPP2R1A protein 150-309aa (BC001537).

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

(1) Rahman,M., Nakayama,K., Rahman,M.T. et al. PPP2R1A mutation is a rare event in ovarian carcinoma across histological subtypes. Anticancer Res. 33 (1), 113-118 (2013)

34783905

BC001537

65,309 Da

AMER1 Mutants Destabilize The Destruction Complex Pathway (1127621); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1127616); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1127617); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1127620); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1127618); Activated TLR4 Signalling Pathway (106400); Adaptive Immune System Pathway (366160); Adrenergic Signaling In Cardiomyocytes Pathway (908257)

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B /PR72/PR130/PR59 and R5/B /B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family. Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19q13.41. Cellular Component: microtubule cytoskeleton; protein phosphatase type 2A complex; mitochondrion; membrane; cytosol; nucleus; chromosome, pericentric region. Molecular Function: protein binding; protein heterodimerization activity; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity; antigen binding. Biological Process: regulation of cell adhesion; second-messenger-mediated signaling; mitotic nuclear envelope reassembly; fibroblast growth factor receptor signaling pathway; apoptosis; RNA splicing; organelle organization and biogenesis; regulation of Wnt receptor signaling pathway; protein amino acid dephosphorylation; chromosome segregation; response to organic substance; regulation of transcription, DNA-dependent; regulation of cell differentiation; regulation of growth; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; protein complex assembly; negative regulation of cell growth; mitotic cell cycle; ceramide metabolic process; G2/M transition of mitotic cell cycle; regulation of DNA replication; inactivation of MAPK activity. Disease: Mental Retardation, Autosomal Dominant 36

0.1 mg

230 USD