NPC1L1 Antibody | MyBioSource MBS416874 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NPC1L1 Antibody

catalog :

MBS416874

quantity :

0.1 mL

price :

205 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS416874

products type :

Antibody

products full name :

NPC1L1 Antibody

products short name :

NPC1L1

products name syn :

NPC1L1

other names :

Niemann-Pick C1-like protein 1 isoform 2; Niemann-Pick C1-like protein 1; Niemann-Pick C1-like protein 1; NPC1 (Niemann-Pick disease, type C1, gene)-like 1; NPC1-like 1

products gene name :

NPC1L1

other gene names :

NPC1L1; NPC1L1; NPC11L1

uniprot entry name :

NPCL1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

1332

specificity :

This Polyclonal antibody is directed against human NPC1L1 protein. The product was purified from seurm by protein A chromatography. Expect reactivity with human NPC1L1. Cross-reactivity with NPC1L1 from other sources has not been determined.

purity :

Affinity purified

form :

Liquid

concentration :

1ug/ul

storage stability :

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

tested application :

Western Blot (WB)

app notes :

Recommended dilutions WB:1/2000. Predicted molecular weight: 145kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determiend by the end user.

other info1 :

Immunogen: Synthetic peptide: FLEEMRAFQRRMAGM, corresponding to amino acids 596-610 of 1ï1/4 Human NPC1L1 Niemann Pick C1 Like 1ï1/4% o .

other info2 :

Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.

products description :

NPC1L1 is a Rabbit polyclonal to NPC1L1

ncbi gi num :

156231353

ncbi acc num :

NP_001095118.1

ncbi gb acc num :

NM_001101648.1

uniprot acc num :

Q9UHC9

ncbi pathways :

Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (106111); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Trafficking Of Dietary Sterols Pathway (106113); Vitamin A And Carotenoid Metabolism Pathway (198785)

ncbi summary :

The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

uniprot summary :

Function: Plays a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Acts as a negative regulator of NPC2 and down-regulates its expression and secretion by inhibiting its maturation and accelerating its degradation. Ref.10 Ref.12. Subunit structure: Interacts with RAB11A, MYO5B and RAB11FIP2. Interaction with RAB11A, MYO5B and RAB11FIP2 is required for proper transport to the plasma membrane upon cholesterol depletion. Interacts with NPC2. Ref.11 Ref.12. Subcellular location: Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein . By similarity. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein . By similarity. According to Ref.8, localizes in a subcellular vesicular compartment rich in RAB5. Ref.8. Tissue specificity: Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. Ref.1 Ref.2 Ref.8. Induction: Expression is decreased in Caco-2 cells upon PPARD activation. Ref.9. Post-translational modification: Highly glycosylated . By similarity. Polymorphism: Variations in NPC1L1 gene could be associated with non-response to ezetimibe treatment. Sequence similarities: Belongs to the patched family.Contains 1 SSD (sterol-sensing) domain.

size1 :

0.1 mL

price1 :

205 USD

more info or order :

MyBioSource product webpage