Anti-ERO1L Antibody | MyBioSource MBS416653 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-ERO1L Antibody

catalog :

MBS416653

quantity :

0.1 mL

price :

230 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS416653

products type :

Antibody

products full name :

Anti-ERO1L Antibody

products short name :

ERO1L

other names :

Homo sapiens ERO1-like (S. cerevisiae), mRNA; ERO1-like protein alpha; ERO1-like protein alpha; endoplasmic oxidoreductin-1-like protein; oxidoreductin-1-L-alpha; ERO1-like (S. cerevisiae); Endoplasmic oxidoreductin-1-like protein; Oxidoreductin-1-L-alpha

products gene name :

ERO1L

other gene names :

ERO1L; ERO1L; ERO1A; ERO1-L; ERO1LA; ERO1-alpha; ERO1-L-alpha

uniprot entry name :

ERO1A_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human. Predicted: Mouse, Rat

sequence length :

1814

purity :

Affinity purified

form :

Liquid

concentration :

100ug/100ul

storage stability :

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

ELISA titer: 1:20,000-1:80,000. Western blot analysis: 1:1,000-1:2,000. Immunohistochemistry: 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user.

other info1 :

Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide

other info2 :

Immunogen: Rabbit polyclonal ERO1L (1) antibody was raised against a recombinated human ERO1L protein 37-189aa (BC008674).

products references :

(1) Araki,K. and Nagata,K. Functional in vitro analysis of the ERO1 protein and protein-disulfide isomerase pathway. J. Biol. Chem. 286 (37), 32705-32712 (2011).

ncbi gi num :

14250469

ncbi acc num :

BC008674

ncbi mol weight :

54,393 Da

ncbi pathways :

Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (1127552); Insulin Processing Pathway (105903); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190); Vibrio Cholerae Infection Pathway (83101); Vibrio Cholerae Infection Pathway (513)

uniprot summary :

ERO1L: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT. Stimulated by hypoxia; suggesting that it is regulated via the HIF-pathway. Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum. Enzyme activity is tightly regulated to prevent the accumulation of reactive oxygen species in the endoplasmic reticulum. Reversibly down-regulated by the formation of disulfide bonds between the active site Cys-94 and Cys-131, and between Cys- 99 and Cys-104. Glutathione may be required to regulate its activity in the endoplasmic reticulum. Belongs to the EROs family. Protein type: Secreted, signal peptide; EC 1.8.4.-; Secreted; Oxidoreductase. Chromosomal Location of Human Ortholog: 14q22.1. Cellular Component: endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; endoplasmic reticulum lumen; endoplasmic reticulum. Molecular Function: protein binding; protein disulfide oxidoreductase activity; protein disulfide isomerase activity; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor. Biological Process: extracellular matrix organization and biogenesis; protein maturation via protein folding; chaperone cofactor-dependent protein folding; protein folding; unfolded protein response; protein modification process; response to temperature stimulus; response to reactive oxygen species; cellular protein metabolic process; 4-hydroxyproline metabolic process; cell redox homeostasis; release of sequestered calcium ion into cytosol; brown fat cell differentiation

size1 :

0.1 mL

price1 :

230 USD

more info or order :

MyBioSource product webpage