Caspase-3 Antibody | MyBioSource MBS415884 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Caspase-3 Antibody

catalog :

MBS415884

quantity :

0.1 mL

price :

205 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS415884

products type :

Antibody

products full name :

Caspase-3 Antibody

products short name :

[Caspase-3]

products name syn :

[Caspase-3]

other names :

[caspase-3 preproprotein; Caspase-3; caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; Apopain; Cysteine protease CPP32; CPP-32]

other gene names :

[CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1]

uniprot entry name :

CASP3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, mouse

sequence length :

277

specificity :

This Polyclonal antibody is directed against human Caspase-3 protein. The product was purified from seurm by protein A chromatography. Expect reactivity with human Caspase-3. Cross-reactivity with Caspase-3 from other sources has not been determined.

purity :

Affinity purified

form :

Liquid

concentration :

1ug/ul

storage stability :

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

tested application :

Western Blot (WB)

app notes :

Recommended dilutions Western Blotting: 1/1000 to 1/2000. Predicted molecular weight: 17 kDa, 34kDa. Not yet tested in other applications. Optimal dilutions/Concentrations should be determined by the end user.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Synthetic peptide derived from Human Caspase-3. Antibody Type: Primary Antibody

other info2 :

Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.

products description :

Caspase-3 is a Rabbit polyclonal to Caspase-3

ncbi gi num :

14790119

ncbi acc num :

NP_004337.2

ncbi gb acc num :

NM_004346.3

uniprot acc num :

P42574

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of DNA Fragmentation Factor Pathway (105683); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alpha6-Beta4 Integrin Signaling Pathway (198807); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099)

ncbi summary :

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Ref.8 Ref.15. Catalytic activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp- - with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. Enzyme regulation: Inhibited by isatin sulfonamides. Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Ref.12. Subcellular location: Cytoplasm. Tissue specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Post-translational modification: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. Sequence similarities: Belongs to the peptidase C14A family.

size1 :

0.1 mL

price1 :

205 USD

more info or order :

MyBioSource product webpage