antibody

Anti-HSBP1 Antibody

MBS415488

0.1 mg

230 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry

Antibody

Anti-HSBP1 Antibody

HSBP1

Homo sapiens heat shock factor binding protein 1, mRNA; Heat shock factor-binding protein 1; heat shock factor-binding protein 1; nasopharyngeal carcinoma-associated antigen 13; heat shock factor binding protein 1; Nasopharyngeal carcinoma-associated antigen 13; NPC-A-13

HSBP1

HSBP1; HSBP1; NPC-A-13

HSBP1_HUMAN

Polyclonal

Rabbit

Human

533

Affinity purified

Liquid

100ug/100ul

This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB), Immunohistochemistry (IHC)

ELISA titer: 1:20,000-1:80,000. Western blot analysis: 1:500-1:1,000. Immunohistochemistry: 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user.

Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide. Antibody Type: Primary Antibody

Immunogen: Raised against a recombinate human HSBP1 protein 1-76aa (BC007515).

Rabbit polyclonal HSBP1

(1) Liu,X., Xu,L., Liu,Y.,et al. Crystal structure of the hexamer of human heat shock factor binding protein 1. Proteins 75 (1), 1-11 (2009).

38197090

BC007515

8,544 Da

Attenuation Phase Pathway (1127556); Cellular Response To Heat Stress Pathway (1127553); Cellular Responses To Stress Pathway (645258); HSF1 Activation Pathway (1127554); HSF1-dependent Transactivation Pathway (1127555)

The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response. [provided by RefSeq, Jul 2008]

HSBP1: Negative regulator of the heat shock response. Negatively affects HSF1 DNA-binding activity. May have a role in the suppression of the activation of the stress response during the aging process. Belongs to the HSBP1 family. Chromosomal Location of Human Ortholog: 16q23.3. Cellular Component: nucleoplasm; cytoskeleton; nucleus. Molecular Function: transcription corepressor activity. Biological Process: muscle contraction; negative regulation of transcription from RNA polymerase II promoter

0.1 mg

230 USD