protein

Pegylated ovine super leptin antagonist (mutant D23/L39A/D40A/F41A)

MBS400092

0.05 mg

225 USD

Recombinant Protein

Pegylated ovine super leptin antagonist (mutant D23/L39A/D40A/F41A)

Pegylated super leptin antagonist

Ovine pegylated super active leptin antagonist (SOLA) mutant D23L/L39A/D40A/F41A

Escherichia Coli

The sequence of the N-terminal amino acids of the pegylated recombinant super active ovine leptin antagonist could not be determined indicating that the PEG moiety is attached to the N-terminus.

The purity od pegylated recombinant super active ovine leptin antagonist is greater than 95.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

Lyophilized pegylated recombinant super active ovine leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below - 20 degree C. Upon reconstitution of pegylated recombinant super active ovine leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization pegylated recombinant super active ovine leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of pegylated recombinant super active ovine leptin antagonist addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized pegylated recombinant super active ovine leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions. Protein Content: Protein quantitation of the pegylated recombinant super active ovine leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.200 as the extinction coefficient for a 0.1% (1mg/ml) protein solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified pegylated recombinant super active ovine leptin antagonist consists of > 90% monomers as determined by gel-filtration chromatography.

Endotoxin: The endotoxin content of pegylated recombinant super active ovine leptin antagonist is less than 0.01 ng/ug (0.1 EU/ug of protein). Formulation: The pegylated recombinant super active ovine leptin antagonist was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3. Biological Activity: pegylated recombinant super active ovine leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The in vitro activity of pegylated recombinant super active ovine leptin antagonist is 6-8 fold lower than the non-pegylated antagonist but is 15 fold higher as compared to pegylated recombinant super active ovine leptin antagonist

Leptin and leptin muteins

Mono-pegylated (with 20 kDa PEG) recombinant super active ovine leptin antagonist is one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume pegylated recombinant super active ovine leptin antagonist runs on the SGS-Page as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. The half-life in circulation of pegylated recombinant super active ovine leptin antagonist after SC injection was over 20 hours. Recombinant super active ovine leptin antagonist was initially mutated, resulting in D23L/L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques. The pegylation of recombinant super active ovine leptin antagonist is similar to that described in Elinav et al. Endocrinology 150:3083-91 (2009) for pegylated recombinant super active mouse leptin antagonist.

0.05 mg

225 USD