protein

Mouse super leptin antagonist (mutant D23/L39A/D40A/F41A)

MBS400086

0.05 mg

165 USD

Recombinant Protein

Mouse super leptin antagonist (mutant D23/L39A/D40A/F41A)

super leptin antagonist

Mouse super-active leptin antagonist (SMLA)

SMLA

Escherichia Coli

The sequence of the first five N-terminal amino acids of mouse super-active leptin antagonist was determined and was found to be Ala-Val-Pro-Ile-Gln

The purity of mouse super-active leptin antagonist is greater than 98.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

Lyophilized mouse super-active leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -20 degree C. Upon reconstitution of mouse super-active leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization mouse super-active leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized mouse super-active leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions. Protein Content: Protein quantization of mouse super-active leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.200 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified mouse super-active leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.

Endotoxin: The endotoxin content of mouse super-active leptin antagonist is less than 0.02 ng/ug (0.2 EU/ug) of mouse leptin antagonist. Formulation: The mouse super-active leptin antagonist was lyophilized from a concentrated (1 mg/ml) solution with 0.003mM NaHCO3. Biological Activity: mouse super-active leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Mouse super-active leptin antagonist also inhibits various leptin effects in several in vitro bioassays. The inhibitory activity of mouse super-active leptin antagonist was increased 14 to 60 fold as measured by various criteria such as binding properties to human leptin binding domain (hLBD) and in vitro and in vivo bioassays as compared to mouse leptin antagonist.

Leptin and leptin muteins

Recombinant mouse leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, mLEP was mutated, resulting in D23L/L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques.

0.05 mg

165 USD

0.1 mg

215

0.5 mg

610

1 mg

965

5 mg

3575