protein

Ovine super leptin antagonist (mutant D23/L39A/D40A/F41A)

MBS400083

0.05 mg

165 USD

Recombinant Protein

Ovine super leptin antagonist (mutant D23/L39A/D40A/F41A)

super leptin antagonist

Ovine super-active leptin antagonist (SOLA)

SolA; N-methyl-L-tryptophan oxidase

SOLA

solA; MTOX

D4GC14_PANAM

Escherichia Coli

371

The sequence of the first five N-terminal amino acids of recombinant super active ovine leptin antagonist was determined and was found to be Ala-Val-Pro-Ile-Arg

The purity of recombinant super active ovine leptin antagonist is greater than 98.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

Lyophilized recombinant super active ovine leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -20 degree C. Upon reconstitution of recombinant super active ovine leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization recombinant super active ovine leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of recombinant super active ovine leptin antagonist addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized recombinant super active ovine leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 10, not less than 100ug/ml, which can then be further diluted with other aqueous solutions. Protein Content: Protein quantitation of recombinant super active ovine leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.200 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified recombinant super active ovine leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.

Endotoxin: The endotoxin content of recombinant super active ovine leptin antagonist is less than 0.02 ng/ug (0.2 EU/ug) of ovine leptin antagonist. Formulation: The recombinant super active ovine leptin antagonist was lyophilized from a concentrated (1 mg/ml) solution with 0.003mM NaHCO3 adjusted to pH 10. Biological Activity: recombinant super active ovine leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays. The inhibitory activity of recombinant super active ovine leptin antagonist was increased 14 to 60 fold as measured by various criteria such as binding properties to human leptin binding domain (hLBD) and in vitro bioassays as compared to recombinant ovine leptin antagonist.

Leptin and leptin muteins

Recombinant mouse leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, was mutated, resulting in D23L/L39A/D40A/F41A mutant termed recombinant super active ovine leptin antagonist that was purified by proprietary chromatographic techniques.

291617003

YP_003519745.1

NC_013956.2

40,914 Da

0.05 mg

165 USD