Human thrombopoietin (hTPO) | MyBioSource MBS400073 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human thrombopoietin (hTPO)

catalog :

MBS400073

quantity :

0.01 mg

price :

175 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS400073

products type :

Recombinant Protein

products full name :

Human thrombopoietin (hTPO)

products short name :

thrombopoietin

products name syn :

Human thrombopoietin (hTPO)

other names :

thrombopoietin; Thrombopoietin; thrombopoietin; MPL ligand; c-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand

products gene name :

hTPO

other gene names :

THPO; THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1; MGDF; ML; MGDF

uniprot entry name :

TPO_HUMAN

host :

E Coli

sequence length :

353

sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Ala-Pr-Pro.

purity :

Greater than 98.0% as determined by:. (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

form :

Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Recombinant TPO although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Recombinant TPO should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

other info1 :

Solubility: It is recommended to reconstitute the lyophilized Recombinant TPO in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:. 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of TPO as a Reference Standard. Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

other info2 :

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of TPO. Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Biological Activity: Recombinant TPO is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of MO7e cells is less then 1 ng/ml, corresponding to a Specific Activity of 1 x 10 6 IU/mg.

products categories :

Hormones, Cytokines and Growth Factors

products description :

Recombinant Human Thrombopoietin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids and having a molecular mass of 18608 Dalton which comprises the receptor binding domain of the Mpl-ligand protein. Recombinant Human Thrombopoietin is purified by proprietary chromatographic techniques.

ncbi gi num :

1401248

ncbi acc num :

AAB03393.1

uniprot acc num :

P40225

ncbi mol weight :

33,604 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (106028); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Platelet Aggregation (Plug Formation) Pathway (106053); Platelet Activation, Signaling And Aggregation Pathway (106034)

ncbi summary :

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

uniprot summary :

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: extracellular space. Molecular Function: growth factor activity; hormone activity; cytokine activity. Biological Process: platelet activation; cell proliferation; positive regulation of protein kinase B signaling cascade; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation. Disease: Thrombocythemia 1

size1 :

0.01 mg

price1 :

175 USD

size2 :

0.05 mg

price2 :

330

size3 :

0.1 mg

price3 :

515

size4 :

1 mg

price4 :

3155

more info or order :

MyBioSource product webpage