protein

Mouse stem cell factor (mSCF)

MBS400071

0.01 mg

175 USD

Recombinant Protein

Mouse stem cell factor (mSCF)

stem cell factor

stem cell factor, partial; Kit ligand; kit ligand; c-Kit ligand; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor; KIT ligand; Mast cell growth factor; MGF

mSCF

KITLG; KITLG; SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7; MGF; SCF; MGF; SCF; sKITLG

SCF_HUMAN

E Coli

37

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Ile-Cys.

98.0% as determined by:. (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. (d) Gel filtration chromatography under non denaturing conditions.

White lyophilized (freeze-dried) powder.

Lyophilized rmSCF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution rHuSCF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized rHuSCF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions conteining buffers and carrier proteins. Protein Content: Protein content:Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: Less than 2% as determined by gel filtration chromatography under non denaturing conditions.

Endotoxin: Less than 0.02 ng/ug (IEU/ug) of mSCF. Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3. Biological Activity: mSCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is less then 2 ng/ml, corresponding to a Specific Activity of 105 IU/mg.

Hormones, Cytokines and Growth Factors

Recombinant murine SCF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18309 Dalton. Bioactive mouse SCF appears as a non covalent homodimer (Lu et al. (1991) JBC, 266, 8102-8107).

1246100

AAB35922.1

P21583

26,667 Da

Adaptive Immune System Pathway (366160); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (198811); Constitutive Signaling By Aberrant PI3K In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Diseases Of Signal Transduction Pathway (1183518)

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q22. Cellular Component: extracellular space; cytoskeleton; cytoplasm; extracellular region; integral to membrane; plasma membrane. Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; male gonad development; germ cell programmed cell death; signal transduction; cell proliferation; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; innate immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication. Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7

0.01 mg

175 USD

0.05 mg

320

0.1 mg

445

1 mg

2110