protein

Ovine prolactin antagonist R129G

MBS400061

0.05 mg

140 USD

Recombinant Protein

Ovine prolactin antagonist R129G

prolactin antagonist R129G

Ovine prolactin antagonist G129R

prolactin; Prolactin; prolactin; decidual prolactin; prolactin

PRL; PRL; PRL

PRL_HUMAN

E Coli

227

The sequence of the first six N-terminal amino acids of the non-truncated form was determined and was found to be Ala-Thr-ProVal-Cys-Pro.

Greater than 99.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

Lyophilized oPRL G129R although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 oPRL mg/ml and up to 4 mg/ml mM and filter sterilization oPRL can be stored at 4 degree C for several weeks.

Solubility: It is recommended to reconstitute the lyophilized oPRL G129R in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified oPRL G129R (23K)or its truncared analogue consists of > 99% monomers as determined by gel-filtration chromatography.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of oPRL G129R. Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3. Biological Activity: oPRL G129R is devoid of agonistic activity and capable of inhibiting biological activity of oPRL or other lactogenic hormones as evidenced by proliferation assay of Nb2 or other cells. The truncated form is more potent inhibitor.

Growth hormones, prolactins, placental lactogens

Recombinant ovine prolactin, G129R mutein is one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (no published information). It is available in two forms: as full size protein and it its DES 9 aa truncated form from its N-terminus which has higher inhibitory activity.

254675133

NP_001157030.1

NM_001163558.2

P01236

25,876 Da

Amyloids Pathway (366238); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Disease Pathway (530764); ErbB4 Signaling Events Pathway (137991); Glucocorticoid Receptor Regulatory Network Pathway (138014); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077)

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family. Protein type: Secreted; Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: extracellular region. Molecular Function: protein binding; prolactin receptor binding; hormone activity. Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; positive regulation of JAK-STAT cascade; regulation of multicellular organism growth; female pregnancy

0.05 mg

140 USD

0.1 mg

190

1 mg

515

5 mg

1845

10 mg

3155