protein

Human placental growth hormone 20K

MBS400052

0.01 mg

155 USD

Recombinant Protein

Human placental growth hormone 20K

placental growth hormone 20K

Human placental growth hormone 20K

Escherichia Coli

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile.

Greater than 98.0% as determined by:. (a) Analysis by RP-HPLC. (b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 11. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 11.

Lyophilized rHuGH-20K-pl although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization rHuGH-20K-pl can be stored at 4 degree C, pH 11 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized rHuGH-20K-pl in 0.4% NaHCO3 or water adjusted to pH 11, not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:. 1. UV spectroscopy at 280 nm using the absorbency value of 1.14 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH-20K-pl as a Reference Standard. Dimers and Aggregates: Less than 3% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of human GH. Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 11. Biological Activity: PLR'r HuGH-20K is fully biologically active when compared to World Health Organization (WHO) reference standard as evidenced by in vitro bioassay. It is also capable of forming a 1:2 complex with the recombinant human growth hormone receptor extracellular domain (ECD).

Growth hormones, prolactins, placental lactogens

Recombinant Placental Human GH-20K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20498 Dalton. Predicted pI=8.20. rHuGH-20K-pl is almost completely devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary hGHs. rHuGH-20K-pl is purified by proprietary chromatographic techniques.

0.01 mg

155 USD

0.05 mg

240

0.1 mg

330

0.5 mg

920

1 mg

1575