protein

Pegylated mouse leptin

MBS400046

0.1 mg

165 USD

Recombinant Protein

Pegylated mouse leptin

Pegylated leptin

Mouse pegylated leptin

leptin; Leptin; leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor; leptin; Obese protein; Obesity factor

LEP; LEP; OB; OBS; LEPD; OB; OBS

LEP_HUMAN

Escherichia Coli

167

The sequence of the N-terminal amino acids of recombinant mouse pegylated leptin could not be determined indicating that the PEG moiety is attached to the N-terminus.

The purity od recombinant mouse pegylated leptin is greater than 99.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

Lyophilized recombinant mouse pegylated leptin although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 recombinant mouse pegylated leptin mg/ml and up to 2 mg/ml and filter sterilization recombinant mouse pegylated leptin can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of recombinant mouse pegylated leptin addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized recombinant mouse pegylated leptin in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions. Protein Content: Protein quantization of recombinant mouse pegylated leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.200 as the extinction coefficient for a 0.1% (1mg/ml) protein solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified recombinant mouse pegylated leptin consists of > 95% monomers as determined by gel-filtration chromatography.

Endotoxin: Less than 0.05 ng/ug (0.5 IEU/ug) of recombinant mouse pegylated leptin. Formulation: The recombinant mouse pegylated leptin was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3 and the powder contains 35 to 40% protein. Biological Activity: recombinant mouse pegylated leptin is capable of stimulatng proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is only slightly lower than the non-pegylated recombinant mouse leptin but in vivo it has profound weight reducing effect (as compared to the non-pegylated recombinant mouse leptin), resulting mainly from reduced food intake.

Leptin and leptin muteins

Mono-pegylated (with 20 kDa PEG)recombinant mouse leptin is an one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume recombinant mouse pegylated leptin runs on the SGS-Page as 48 kDa protein and in gel-filtration on Superdex 200 as over 100 kDa protein. Recombinant mouse pegylated leptin half-life in circulation after SC injection was over 20 hours. Recombinant mouse leptin was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purification 47, 128 -136 and then pegylated.

4557715

NP_000221.1

NM_000230.2

P41159

18,641 Da

AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Developmental Biology Pathway 477129!!HIF-1-alpha Transcription Factor Network Pathway 138045

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

0.1 mg

165 USD