protein

MTS-tagged ovine leptin

MBS400044

0.1 mg

190 USD

Recombinant Protein

MTS-tagged ovine leptin

MTS-tagged ovine leptin

leptin; Leptin; leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor; leptin; Obese protein; Obesity factor

LEP; LEP; OB; OBS; LEPD; OB; OBS

LEP_HUMAN

E Coli

167

The sequence of the first five N-terminal amino acids ovine recombinant leptin was determined and was found to be Ala-Val-Leu-Leu-Pro.

The purity of MYS-ovine recombinant leptin is greater than 98.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. (c) Gel-filtration chromatography under non denaturing conditions.

White lyophilized (freeze-dried) powder.

Lyophilized MTS-ovine recombinant leptin although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of MTS-ovine recombinant leptin at > 0.1 hLEP mg/ml and up to 2 mg and filter sterilization hLEP can be stored at +4 degree C.

Solubility: It is recommended to reconstitute the lyophilized MTS-ovine recombinant leptin in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation of MTS-ovine recombinant leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.18 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified MTS-ovine recombinant leptin (17.5 K) consists of > 96% monomers as determined by gel-filtration chromatography.

Endotoxin: The endotoxin content of MTS-ovine recombinant leptin is less than 0.1 ng/ug (IEU/ug). Formulation: The MTS-ovine recombinant leptin was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. Biological Activity: MTS-ovine recombinant leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. In vivo experiments has shown potency equal but not higher that non tagged ovine leptin (A. Gertler, unpublished data).

Leptin and leptin muteins

Recombinant ovine MTS-leptin, one polypeptide chain containing 157 amino and (extra 10 amino acids of the membrane translocating sequence Val-Leu-Leu-Pro-Val-Leu-Leu-Ala-Ala-Pro) derived from Kaposi virus and additional Ala at N-terminus acids. The resulting molecular mass of is ~ 17.5 kDa, MTS-ovine leptin was purified by proprietary chromatographic techniques.

4557715

NP_000221.1

NM_000230.2

P41159

18,641 Da

AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Developmental Biology Pathway 477129!!HIF-1-alpha Transcription Factor Network Pathway 138045

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

0.1 mg

190 USD