product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Human leptin binding domain
catalog :
MBS400041
quantity :
0.05 mg
price :
270 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS400041
products type :
Recombinant Protein
products full name :
Human leptin binding domain
products short name :
leptin binding domain
host :
E Coli
sequence :
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile
purity :
Purity:Greater than 99.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
form :
The protein was lyophilized from a concentrated (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150 mM NaCl and 10-fold (w/w) excess of glycine. This formulation allows full solubilization by adding water and gentle mixing and fully preserves
storage stability :
Lyophilized hLBD should be stored desiccated below -18C. Upon reconstitution at > 0.1 hLBD mg/ml and up to 40.5 mg/ml mM and filter sterilization hLBD can be stored at 4C for several months. Sterile solutions at 0.5 mg/ml or less are stable at +4C for several months.
other info1 :
Dimers and Aggregates: The purified hLBD (24K) consists of > 95% monomers as determined by gel-filtration chromatography. Biological Activity: MyBioSource's hLBDL is fully biologically active as evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
other info2 :
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of hLBD . Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 9.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Solubility: It is recommended to reconstitute the lyophilized hLBD in sterile water adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
products categories :
Soluble receptors
products description :
Recombinant human leptin binding domain (hLBD), is one polypeptide chain containing 208 amino acids and an additional Ala at N-terminus acids. It consists of the cytokine binding domain of leptin receptor (amino acids 428-635 of human leptin receptor and having a molecular mass of ~ 24.5 kDa, It was purified by proprietary chromatographic techniques (see Sandowski et al. J. Biol. Chem. (2002) 277(48):46304-9.
size :
0.05 mg
price :
270 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

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