MyBioSource

protein

Ovine leptin antagonist (mutant L39A/D40A/F41A/I42A)

MBS400040

0.05 mg

160 USD

MBS400040

Recombinant Protein

Ovine leptin antagonist (mutant L39A/D40A/F41A/I42A)

leptin antagonist (mutant L39A/D40A/F41A/I42A)

leptin; Leptin; leptin; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); leptin; Obese protein; Obesity factor

LEP; LEP; OB; OBS; LEPD; OB; OBS

LEP_HUMAN

E Coli

167

The sequence of ovine recombinant leptin antagonist of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg

The purity of ovine recombinant leptin antagonist is greater than 98.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

The ovine recombinant leptin antagonist was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Physical Appearance: White lyophilized (freeze-dried) powder.

Lyophilized ovine recombinant leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution of ovine recombinant leptin antagonist at > 0.1 oLEP mutant mg/ml and up to 2 mg/ml and filter sterilization ovine recombinant leptin antagonist can be stored at +4C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of ovine recombinant leptin antagonist addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Dimers and Aggregates: The purified ovine recombinant leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography. Biological Activity: MyBioSource's ovine recombinant leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of ovine leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

Endotoxin: The endotoxin of ovine recombinant leptin antagonist is less than 0.1 ng/ug (IEU/ug) of ovine leptin antagonist. Protein Content: Protein quantitation of ovine recombinant leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Solubility: It is recommended to reconstitute the lyophilized ovine recombinant leptin antagonist in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Leptin related products; Leptin and leptin muteins

Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, ovine leptin was mutated, resulting in L39A/D40A/F41A/I42A mutant was purified by proprietary chromatographic techniques. Preparation of leptin antagonists was published - Niv-Spector et al, Biochem. J 291;221-230 (2005).

4557715

NP_000221.1

NM_000230.2

P41159

18,641 Da

AMPK Signaling Pathway 198868!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Developmental Biology Pathway 477129!!HIF-1-alpha Transcription Factor Network Pathway 138045!!Incretin Synthesis, Secretion, And Inactivation Pathway 187170!!Jak-STAT Signaling Pathway 83077

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Subunit structure: Interacts with SIGLEC6. Ref.11. Subcellular location: Secreted. Involvement in disease: Leptin deficiency (LEPD) [MIM:614962]: A rare disease characterized by low levels of serum leptin, severe hyperphagia and intractable obesity from an early age.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.16. Sequence similarities: Belongs to the leptin family.

0.05 mg

160 USD