protein

Pegylated rat leptin antagonist (mutant L39A/D40A/F41A)

MBS400038

0.05 mg

225 USD

Recombinant Protein

Pegylated rat leptin antagonist (mutant L39A/D40A/F41A)

Pegylated leptin antagonist

Rat pegylated leptin antagonist (mutant L39A/D40A/F41A)

leptin; Leptin; leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor; leptin; Obese protein; Obesity factor

LEP; LEP; OB; OBS; LEPD; OB; OBS

LEP_HUMAN

Escherichia Coli

167

The sequence of N-terminal amino acids of pegylated rat leptin antagonist could not be determined indicating that the PEG is attached to the N-terminus.

The purity of pegylated rat leptin antagonist is greater than 99.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

Lyophilized pegylated rat leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of pegylated rat leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization pegylated rat leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of pegylated rat leptin antagonist addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the pegylated rat leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions. Protein Content: Protein quantitation of pegylated rat leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution of protein at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified pegylated rat leptin antagonist (16K) consists of > 99% monomers as determined by gel-filtration chromatography.

Endotoxin: Less than 0.05 ng/ug (EU/ug) of pegylated rat leptin antagonist. Formulation: The recombinant pegylated rat leptin antagonist was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3. Biological Activity: pegylated rat leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The in vitro activity of pegylated rat leptin antagonist is 5-6 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist), resulting mainly from increased food intake.

Leptin and leptin muteins

Mono-pegylated (with 20 kDa PEG) recombinant rat leptin antagonist is one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume pegylated recombinant rat leptin antagonist runs on the SGS-Page as 48 kDa protein and on gel-filtration Superdex 200 column as over 100 kDa protein. The half-life of recombinant rat leptin antagonist in circulation after SC injection was over 20 hours. Recombinant rat leptin was initially mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purification 47, 128 -136.

4557715

NP_000221.1

NM_000230.2

P41159

18,641 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); HIF-1-alpha Transcription Factor Network Pathway (138045)

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family. Protein type: Cell development/differentiation; Secreted; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q31.3. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: growth factor activity; peptide hormone receptor binding; hormone activity. Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; female pregnancy; glucose homeostasis; negative regulation of appetite; positive regulation of luteinizing hormone secretion; positive regulation of tyrosine phosphorylation of Stat3 protein; response to insulin stimulus; response to vitamin E; regulation of cholesterol absorption; positive regulation of MAPKKK cascade; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; placenta development; central nervous system neuron development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; bile acid metabolic process; eating behavior; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; glycerol biosynthetic process; insulin secretion; response to hypoxia; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; positive regulation of follicle-stimulating hormone secretion; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis. Disease: Leptin Deficiency

0.05 mg

225 USD

0.1 mg

330

0.5 mg

1140

1 mg

1845

5 mg

7085