protein

Rat leptin antagonist (mutant L39A/D40A/F41A)

MBS400037

0.05 mg

145 USD

Recombinant Protein

Rat leptin antagonist (mutant L39A/D40A/F41A)

leptin antagonist

leptin

Escherichia Coli

167

The sequence of the first five N-terminal amino acids of recombinant rat leptin antagonist was determined and was found to be Ala-Val-Pro-Ile-Gln

The purity of recombinant rat leptin antagonist is greater than 98.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

0.85mg/ml

Lyophilized recombinant rat leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of recombinant rat leptin antagonist at > 0.1 rLEP mutant mg/ml and up to 2 mg/ml and filter sterilization recombinant rat leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of recombinant rat leptin antagonist addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized recombinant rat leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions. Protein Content: Protein quantitation of recombinant rat leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified recombinant rat leptin antagonist (16K) consists of > 97% monomers as determined by gel-filtration chromatography.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of recombinant rat leptin antagonist. Formulation: Recombinant rat leptin was lyophilized from a concentrated (0.85mg/ml) solution with 0.003mM NaHCO3. Biological Activity: recombinant rat leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

Leptin and leptin muteins

Recombinant rat leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant rat leptin was mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques, according to Salomon et al (2006) Protein Expression and PuriWcation 47, 128 -136.

4557715

NP_000221.1

NM_000230.2

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); HIF-1-alpha Transcription Factor Network Pathway (138045)

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family. Protein type: Secreted; Cell development/differentiation; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q31.3. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: peptide hormone receptor binding; growth factor activity; hormone activity. Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; female pregnancy; glucose homeostasis; positive regulation of luteinizing hormone secretion; negative regulation of appetite; positive regulation of tyrosine phosphorylation of Stat3 protein; response to insulin stimulus; response to vitamin E; regulation of cholesterol absorption; positive regulation of MAPKKK cascade; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; bile acid metabolic process; eating behavior; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; glycerol biosynthetic process; insulin secretion; response to hypoxia; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; positive regulation of follicle-stimulating hormone secretion; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis. Disease: Leptin Deficiency

0.05 mg

145 USD

0.1 mg

175

0.5 mg

515

1 mg

785

5 mg

2890