protein

Human leptin antagonist (mutant L39A/D40A/F41A)

MBS400034

0.05 mg

145 USD

Recombinant Protein

Human leptin antagonist (mutant L39A/D40A/F41A)

leptin antagonist

Human leptin antagonist (mutant L39A/D40A/F41A)

leptin; Leptin; leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor; leptin; Obese protein; Obesity factor

LEP; LEP; OB; OBS; LEPD; OB; OBS

LEP_HUMAN

Escherichia Coli

167

The sequence of the first five N-terminal amino acids of recombinant human leptin antagonist was determined and was found to be Ala-Val-Pro-Ile-Gln

The purity of recombinant human leptin antagonist is greater than 98.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

Lyophilized recombinant human leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at of recombinant human leptin antagonist > 0.1mg/ml and up to 2 mg/ml and filter sterilization human recombinant leptin antagonist can be stored at +4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of recombinant human leptin antagonist addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized recombinant human leptin antagonist in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions preferably in presence of carrier protein. Protein Content: Protein quantitation of recombinant human leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified recombinant human leptin antagonist (16K) consists of > 97% monomers as determined by gel-filtration chromatography.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of recombinant human leptin antagonist. Formulation: The recombinant protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Biological Activity: recombinant human leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

Leptin and leptin muteins

Recombinant human leptin is one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant human leptin was mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques. Preparation of leptin antagonists was recently published in Biochemical Journal (Niv-Spector et al, Biochem. J 291;221-230 (2005).

4557715

NP_000221.1

NM_000230.2

P41159

18,641 Da

AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Developmental Biology Pathway 477129!!HIF-1-alpha Transcription Factor Network Pathway 138045

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

0.05 mg

145 USD