protein

Porcine leptin

MBS400033

0.1 mg

190 USD

Recombinant Protein

Porcine leptin

leptin

leptin; Leptin; leptin; obesity factor; leptin; Obesity factor

Lep; Lep; OB; obese; Ob

LEP_RAT

E Coli

167

The sequence of the first five N-terminal amino acids of recombinant porcine leptin was determined and was found to be Ala-Val-Pro-Ile-Trp

The purity of recombinant porcine leptin is greater than 98.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. (c) Gel-filtration chromatography under non denaturing conditions.

White lyophilized (freeze-dried) powder.

Lyophilized recombinant porcine leptin although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of recombinant porcine leptin at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization recombinant porcine leptin can be stored at +4 degree C.

Solubility: It is recommended to reconstitute the lyophilized recombinant porcine leptin in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation of recombinant porcine leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.57 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified recombinant porcine leptin (16K) consists of > 97% monomers as determined by gel-filtration chromatography.

Endotoxin: The endotoxin content of recombinant porcine leptin is less than 0.1 ng/ug (IEU/ug) of porcine leptin. Formulation: The recombinant porcine leptin was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. Biological Activity: recombinant porcine leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

Leptin and leptin muteins

Recombinant porcine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Recombinant porcine leptin was purified by proprietary chromatographic techniques, see Raver et al. Prot. Express. Purif. 19, 30-40 (2000).

6981148

NP_037208.1

NM_013076.3

P50596

18,866 Da

AMPK Signaling Pathway (989832); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198479); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Incretin Synthesis, Secretion, And Inactivation Pathway (1177199); Jak-STAT Signaling Pathway (83469); Jak-STAT Signaling Pathway (488)

involved in regulation of body weight, and may have a role in angiogenesis [RGD, Feb 2006]

0.1 mg

190 USD

0.5 mg

500

1 mg

785

5 mg

2555

10 mg

4480