protein

Mouse interleukin 3

MBS400021

0.01 mg

175 USD

Recombinant Protein

Mouse interleukin 3

interleukin 3

Mouse interleukin 3 (mIL-3)

interleukin-3; Interleukin-3; interleukin-3; P-cell-stimulating factor; hematopoietic growth factor; mast cell growth factor; multipotential colony-stimulating factor; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor

mIL-3

IL3; IL3; IL-3; MCGF; IL-3; MCGF

IL3_CANFA

E Coli

143

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Asp-Thr-His-Arg.

Greater than 98.0% as determined by:. (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. (d) Gel filtration chromatography under non-denaturing conditions.

Lyophylized white powder

Lyophilized IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180 degree C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized IL-3 in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions containing carrier protein such as BSA, HSA or similar. Protein Content: Protein quantitation was carried out by two independent methods:. 1. UV spectroscopy at 280 nm using the absorbency value of 0.227 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Murine IL-3 as a Reference Standard. Dimers and Aggregates: Less than 2% as determined by gel filtration under non-denaturing conditions.

Endotoxin: Less than 0.05 ng/ug (0.5 EU/ug) of Recombinant IL-3. Formulation: Interleukin-3 is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3. Biological Activity: murine IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant proliferation of murine Baf/3 cells.

Hormones, Cytokines and Growth Factors

Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases. Recombinant murine IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton. Recombinant Murine IL-3 is purified by proprietary chromatographic techniques.

62078415

NP_001013857.1

NM_001013835.1

Q9BDX4

16,499 Da

Apoptosis Pathway (84110); Apoptosis Pathway (470); Asthma Pathway (84166); Asthma Pathway (532); Cytokine-cytokine Receptor Interaction Pathway (84101); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198591); Fc Epsilon RI Signaling Pathway (84132); Fc Epsilon RI Signaling Pathway (493); Hematopoietic Cell Lineage Pathway (84128)

0.01 mg

175 USD

0.05 mg

320

0.1 mg

445

1 mg

2110