protein

Rabbit growth hormone soluble receptor (rbGH binding protein)

MBS400016

0.05 mg

330 USD

Recombinant Protein

Rabbit growth hormone soluble receptor (rbGH binding protein)

growth hormone soluble receptor

Rabbit growth hormone soluble receptor (so called GH binding protein)

Growth hormone receptor; Growth hormone receptor; growth hormone receptor; GH receptor; growth hormone binding protein; serum binding protein; somatotropin receptor; growth hormone receptor; Somatotropin receptor

GHR; GHR; GHBP; GHIP; GH receptor; GH-binding protein; GHBP

GHR_HUMAN

Escherichia Coli

638

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.

Greater than 98.0% as determined by:. (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

White lyophilized (freeze-dried) powder.

Lyophilized rbGHBP although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 rbGHBP mg/ml and filter sterilization hGHBP can be stored at 4 degree C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized rbGHBP in sterile 0.4% NaHCO3 adjusted to pH 10, not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Dimers and Aggregates: The purified rbGHBP (28K) consists of > 98% monomers as determined by gel-filtration chromatography.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of rbGHBP (28 K). Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Biological Activity: hGHBP is fully biologically active as evidenced by its ability of forming 2:1 complex with non-primate GHs.

Soluble receptors

Recombinant rbGHBP, one polypeptide chain containing 248 amino acids and having a molecular mass of ~ 28 kDa, Rabbit GHBP was purified by proprietary chromatographic techniques, (see Sakal et al. Prep Biochem Biotechnol. 30:107-23, 2000).

121180

P10912.1

P10912

69,237 Da

Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Endochondral Ossification Pathway 198812!!Growth Hormone Receptor Signaling Pathway 477128!!Immune System Pathway 106386!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488!!Neuroactive Ligand-receptor Interaction Pathway 83053!!Neuroactive Ligand-receptor Interaction Pathway 462

This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

0.05 mg

330 USD