protein

Human glial derived neorotropic factor (hGDNF)

MBS400009

0.01 mg

200 USD

Recombinant Protein

Human glial derived neorotropic factor (hGDNF)

glial derived neorotropic factor

glial cell line-derived neurotrophic factor isoform 4 preproprotein; Glial cell line-derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor; glial cell derived neurotrophic factor; Astrocyte-derived trophic factor; ATF

hGDNF

GDNF; GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF; hGDNF; ATF

GDNF_HUMAN

E Coli

202

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.

Greater than 98.0% as determined by:. (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Glial Derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GDNF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions containing carrier proteins such as BSA or HSA.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of bPL . Formulation: GDNF was lyophilized after dialysis against 10 mM sodium citrate and 150mM NaCl. Biological Activity: The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml.

Hormones, Cytokines and Growth Factors

GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Glial Derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton. GDNF is purified by proprietary chromatographic techniques.

299473781

NP_001177398.1

NM_001190469.1

P39905

18,123 Da

Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!NCAM Signaling For Neurite Out-growth Pathway 105689!!NCAM1 Interactions Pathway 105697!!Signaling Events Regulated By Ret Tyrosine Kinase Pathway 137915!!Spinal Cord Injury Pathway 739007

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

0.01 mg

200 USD