protein

Mouse soluble CD40 Ligand (mSCD40L)

MBS400004

0.01 mg

155 USD

Recombinant Protein

Mouse soluble CD40 Ligand (mSCD40L)

soluble CD40 Ligand

Mouse soluble CD40 Ligand (hsCD40-L)

CD40 ligand; CD40 ligand; CD40 ligand; CD40 antigen ligand; CD40-L; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; TRAP

mSCD40L

CD40LG; CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40L; TNFSF5; TRAP; CD40-L; TRAP

CD40L_HUMAN

261

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Lys-Gly-Asp.

Greater than 98.0% as determined by:. (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized CD40L although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CD40L should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized CD40L in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions containing carrier protein. Protein Content: Protein quantitation was carried out by two independent methods:. 1. UV spectroscopy at 280 nm using the absorbency value of 1.1 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of soluble CD40L as a Reference Standard. Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of soluble CD40L. Formulation: CD40L is lyophilized from a sterile concentrated solution (1mg/ml) with 10mM Sodium Phosphate pH=7.5. Biological Activity: CD40L is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml.

Hormones, Cytokines and Growth Factors

CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. Recombinant Human CD40L produced in E.Coli is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16308 Dalton. Recombinant CD40L is purified by proprietary chromatographic techniques.

4557433

NP_000065.1

NM_000074.2

P29965

29,274 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD40/CD40L Signaling Pathway (138061); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family. Protein type: Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: Xq26. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding. Biological Process: B cell proliferation; platelet activation; leukocyte adhesion; regulation of immune response; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis. Disease: Immunodeficiency With Hyper-igm, Type 1

0.01 mg

155 USD

0.05 mg

295

0.1 mg

475

1 mg

2380