protein

Sphingosine Kinase 2, Long Form, Human

MBS396454

0.05 mg

195 USD

Peptide

Sphingosine Kinase 2, Long Form, Human

Sphingosine Kinase 2, Long Form, Human

Sphk2-L; N-terminal-extended Sphingosine Kinase 2

sphingosine kinase 2 isoform a; Sphingosine kinase 2; sphingosine kinase 2; sphingosine kinase type 2; sphingosine kinase 2

SPHK2; SPHK2; SK 2; SK-2; SPK 2; SPK-2; SK 2; SPK 2

SPHK2_HUMAN

IgG

Rabbit

654

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles. Products are stable for one year from purchase when stored properly.

Antibody Function Blocking

Comments: Blocking peptide for use with Human Sphingosine Kinase 2, Long Form antibodies.

Instruction: Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37 Deg. C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western Blot. Example: 10ul or 10ug of Exalpha’s rabbit anti-human Sphingosine Kinase 2 , Long form is added to 10ug of blocking peptide for a total volume of 20 ul. The mixture is allowed to incubate for 20 minutes at 37 Deg. C prior to dilution in suitable buffer (for Western Blot, etc.).

Blocking Peptide; Protein Kinases

Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.

323462187

NP_001191088.1

NM_001204159.2

Q9NRA0

69,217 Da

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Ceramide Signaling Pathway (138023); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590); IL12-mediated Signaling Events Pathway (137922); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Signal Transduction Of S1P Receptor Pathway (198819); Sphingolipid Metabolism Pathway (760636)

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. Ref.8. Catalytic activity: ATP + sphinganine = ADP + sphinganine 1-phosphate. Ref.1ATP + sphingosine = ADP + sphingosine 1-phosphate. Ref.1. Cofactor: Magnesium . By similarity. Enzyme regulation: Inhibited by sulfatide. Ref.8. Subcellular location: Isoform 1: Cytoplasm. Membrane Ref.6 Ref.8. Isoform 2: Lysosome membrane Ref.6 Ref.8. Sequence similarities: Contains 1 DAGKc domain. Biophysicochemical propertiesKinetic parameters:KM=3.4 uM for sphingosine Ref.1pH dependence:Optimum pH is 7.5.

0.05 mg

195 USD