protein

Sulf2

MBS396388

0.05 mg

195 USD

Peptide

Sulf2

Sulf2

Extracellular Sulfatase-2; HSulf-2

extracellular sulfatase Sulf-2 isoform a; Extracellular sulfatase Sulf-2; extracellular sulfatase Sulf-2; sulfatase 2

Sulf2

SULF2; SULF2; HSULF-2; KIAA1247; hSulf-2

SULF2_HUMAN

870

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles. Products are stable for one year from purchase when stored properly.

Antibody Function Blocking

Comment: Blocking peptide for use with Sulf-2 antibodies. Instruction: Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37 deg C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western blot. Example: 10 ?l or 10 ?g of Exalpha's rabbit anti-Sulf-2 is added to 10 ?g of blocking peptide for a total volume of 20 ?l. The mixture is allowed to incubate for 20 minutes at 37 deg C prior to dilution in suitable buffer (for Western blot, etc.).

Blocking Peptide

Sulf-2 exhibits arylsulfatase activity and highly specific endoglucosamine -6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Heparan sulfate proteoglycans act as co-receptors for numerous heparinbinding growth factors and cytokines and are key regulators of cell signaling. Growth factor binding to heparan sulfate results in mitogenic activity only when specific structural features are present within the heparan sulfate chains. These features include sulfation at specific positions within a disaccharide (N, 2-O, 3-O, 6-O) by the enzymes that regulate heparan sulfate synthesis inside the Golgi. Following synthesis and expression, heparan sulfate can be structurally and functionally modified within the extracellular compartment. Enzymes known to have these effects are heparanase, which cleaves heparan sulfate chains into smaller biologically active fragments, and the heparan sulfate 6-Oendosulfatases (Sulfs). The Sulfs represent a new and novel family of enzymes that are secreted via the Golgi and subsequently localized to the cell surface or may be released into the extracellular matrix. Sulf-1 enzyme is required for Wnt-mediated signaling in developing quail muscle. Sulf1 was shown (in quial) to restore bone morphogenetic protein signaling in cells by releasing the functional inhibitor, Noggin, from cell surfaces. Quail Sulf1 can also inhibit growth factor signaling, the removal of the 6-O-sulfation is required for the formation of the FGF HS FR1c ternary complex blocking FGF2 signaling. . Analysis of human tumor tissue and tumor cell lines suggests that HSulf-1 is misregulated in cancers. HSulf-1 is found in a variety of normal tissues but is down-regulated in tumor cell lines originating from ovarian, breast, pancreatic, renal, and hepatocellular carcinoma.

240255483

NP_001155313.1

NM_001161841.1

Q8IWU5

100455 Da

Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]

Function: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Cofactor: Binds 1 calcium ion per subunit . By similarity. Subcellular location: Endoplasmic reticulum . By similarity. Golgi apparatus Golgi stack . By similarity. Cell surface . By similarity. Note: Also localized on the cell surface . By similarity. Tissue specificity: Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. Post-translational modification: The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity . By similarity. Sequence similarities: Belongs to the sulfatase family. Biophysicochemical propertiespH dependence:Optimum pH is 7.0-8.0. Sequence caution: The sequence AAH20962.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA86561.2 differs from that shown. Reason: Erroneous initiation. The sequence CAB61349.1 differs from that shown. Reason: Erroneous initiation.

0.05 mg

195 USD