antibody

Sphingosine Kinase 2, Long Form, Human

MBS395712

10 Miniblots

440 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

Sphingosine Kinase 2, Long Form, Human

Sphingosine Kinase 2, Long Form, Human

Sphk2-L; N-terminal-extended Sphingosine Kinase 2

sphingosine kinase 2 isoform a; Sphingosine kinase 2; sphingosine kinase 2; sphingosine kinase type 2; sphingosine kinase 2

SPHK2; SPHK2; SK 2; SK-2; SPK 2; SPK-2; SK 2; SPK 2

SPHK2_HUMAN

Polyclonal

IgG

Rabbit

Human

654

Antigen Immunoaffiinity Purification

Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide. Affinity Purified

Product should be stored at -70 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot (WB), ELISA (EIA)

Immunogen: Synthetic peptide derived from the N-terminal extension of the Sphingosine Kinase 2-L protein.

Positive Control: Jurkat lysate used as positive control.

Antigen Immunoaffinity Purified Polyclonal; Protein Kinases

Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.

323462187

NP_001191088.1

NM_001204159.2

Q9NRA0

69,217 Da

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Ceramide Signaling Pathway (138023); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590); IL12-mediated Signaling Events Pathway (137922); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Signal Transduction Of S1P Receptor Pathway (198819); Sphingolipid Metabolism Pathway (760636)

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. Ref.8. Catalytic activity: ATP + sphinganine = ADP + sphinganine 1-phosphate. Ref.1ATP + sphingosine = ADP + sphingosine 1-phosphate. Ref.1. Cofactor: Magnesium . By similarity. Enzyme regulation: Inhibited by sulfatide. Ref.8. Subcellular location: Isoform 1: Cytoplasm. Membrane Ref.6 Ref.8. Isoform 2: Lysosome membrane Ref.6 Ref.8. Sequence similarities: Contains 1 DAGKc domain. Biophysicochemical propertiesKinetic parameters:KM=3.4 uM for sphingosine Ref.1pH dependence:Optimum pH is 7.5.

10 Miniblots

440 USD