antibody

M13 bacteriophage (gp3)

MBS395351

1 mg

385 USD

monoclonal

mouse

nonconjugated

E1

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

Antibody

M13 bacteriophage (gp3)

M13 bacteriophage (gp3)

Coat protein A; G3P; Minor coat protein

structural protein; Attachment protein G3P; attachment protein; Gene 3 protein; G3P; Minor coat protein

III; III; G3P

G3P_BPM13

Monoclonal

IgG2a

E1

Mouse

424

Protein A/G Chromatography

Provided as solution in phosphate buffered saline with 0.08% sodium azide

1mg/ml

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles. Products are stable for one year from purchase when stored properly. Shippin: Ship at ambient temperature, freeze upon arrival.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells. Conjugate: Unconjugated

ACCESSION NUMBER: P69168, M13 Phage. Comments: Antibody specific for the gp3 protein. Antibody can be used for immunohistochemistry, Western blot (1-5 ug/ml), Flow cytometry (1 ug/10 6 cells) and ELISA. Optimal concentration should be evaluated by serial dilutions.

Monoclonal Antibody; Phage Display

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

17426224

NP_510891.1

NC_003287.2

P03662

Function: Plays essential roles both in the entry of the viral genome into the bacterial host and in the budding process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host coreceptor tolA induces injection of the viral DNA into the host cytoplasm. In the budding process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain. Subunit structure: Interacts with G6P; this interaction is required for proper integration of G3P and G6P into the virion. Interacts with G8P . By similarity. Interacts with host tolA . By similarity. Ref.2. Subcellular location: Virion. Host membrane; Single-pass type I membrane protein . Potential. Note: Prior to assembly, G3P is found associated with the bacterial host inner membrane. There are about five copies of this protein per mature phage that are located on the head side of the filamentous virion. Domain: Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain forms a complex with the C-terminal domain of tolA at later stages of the infection process. N1 is connected to N2 by a flexible glycine-rich linker on the phage. The C-terminal domain is required for release of viral particles from the host bacterial membrane and proper integration of G3P and G6P proteins in the virion. Post-translational modification: In one of the crystallographic structures Trp-39 is oxidized to 1',2'-dihydro-2'-oxotryptophan (Ref.3). Sequence similarities: Belongs to the inovirus G3P protein family.

1 mg

385 USD