antibody

Interleukin-6 (IL-6)

MBS395308

0.5 mg

365 USD

monoclonal

mouse

nonconjugated

AS16

immunohistochemistry, neutralization, immunoprecipitation

Antibody

Interleukin-6 (IL-6)

Interleukin-6 (IL-6)

IL6; Interleukin 6; beta 2 Interferon

interleukin-6; IL6 protein; interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor; interleukin 6 (interferon, beta 2)

IL-6

IL6; IL6; HGF; HSF; BSF2; IL-6; IFNB2

Q75MH2_HUMAN

Monoclonal

IgG1

AS16

Mouse

Human

212

Protein A/G Chromatography

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles. Antibodies are stable for on year from purchase if stored frozen.

Immunoprecipitation (IP), Immunohistochemistry (IHC), Neutralization

This antibody has been used at a 200-fold excess to neutralize more than 95% human IL-6. Immunoprecipitation: This antibody has been used under appropriate conditions to immunoprecipitate >90% hIL-6 in solution using a double-antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980). Immunohistochemistry: THis antibody has been used at 1-10 ug/ml to stain hIL-6 expressed in mononuclear cells and liver tissue, following fixation with 4% paraformaldehyde in PBS for 10 minutes at 4 deg C. plus 0.1% saponin 20 minutes at RT.

Immunogen: Human recom- binant interleukin 6 (hIL-6).

Positive Control: Specificity: Recognizes human IL-6. recognize mouse IL-6 or a panel of other human cytokines.

Monoclonal Antibody; Cytokines

IL-6 (MP5-20F3) RTXMS; Unconjugated Monoclonal antibody specific to Mouse IL-6. This antibody is validated for use in Flow Cytometry, Immunoassay (ELISA). Anti-IL-6 recognizes the expressed product of the Il6 gene.

10834984

NP_000591.1

NM_000600.3

Q75MH2

23,718 Da

ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051)

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]

IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7p21. Cellular Component: extracellular space; cytoplasm; extracellular region; interleukin-6 receptor complex; external side of plasma membrane. Molecular Function: protein binding; interleukin-6 receptor binding; growth factor activity; cytokine activity. Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; bone remodeling; glucose homeostasis; positive regulation of tyrosine phosphorylation of Stat3 protein; response to caffeine; activation of NF-kappaB transcription factor; muscle maintenance; response to antibiotic; regulation of cell shape; monocyte chemotaxis; positive regulation of T-helper 2 cell differentiation; positive regulation of acute inflammatory response; negative regulation of gluconeogenesis; acute-phase response; positive regulation of T cell proliferation; cell growth; response to electrical stimulus; defense response to virus; neurite development; response to drug; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of interleukin-6 production; negative regulation of fat cell differentiation; defense response to protozoan; response to amino acid stimulus; positive regulation of chemokine production; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; response to heat; cell redox homeostasis; positive regulation of B cell activation; negative regulation of protein kinase activity; neutrophil apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of muscle development; positive regulation of translation; negative regulation of hormone secretion; positive regulation of smooth muscle cell proliferation; neutrophil mediated immunity; negative regulation of caspase activity; regulation of circadian sleep/wake cycle, non-REM sleep; defense response to Gram-negative bacterium; response to insulin stimulus; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to peptidoglycan; positive regulation of cell proliferation; hepatic immune response; inflammatory response; aging; negative regulation of chemokine biosynthetic process; response to nutrient levels; cytokine and chemokine mediated signaling pathway; positive regulation of immunoglobulin secretion; endocrine pancreas development; regulation of angiogenesis; positive regulation of peptidyl-serine phosphorylation; humoral immune response; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; response to cold; positive regulation of neuron differentiation; positive regulation of DNA replication; positive regulation of transmission of nerve impulse. Disease: Rheumatoid Arthritis, Systemic Juvenile; Arteriovenous Malformations Of The Brain; Kaposi Sarcoma, Susceptibility To; Inflammatory Bowel Disease 1

0.5 mg

365 USD