product summary
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company name :
MyBioSource
product type :
antibody
product name :
DNA Adenine Methylase
catalog :
MBS395227
quantity :
0.1 mg
price :
315 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RB1
reactivity :
Escherichia coli str. K-12 substr. MG1655
application :
western blot
more info or order :
product information
catalog number :
MBS395227
products type :
Antibody
products full name :
DNA Adenine Methylase
products short name :
DNA Adenine Methylase
products name syn :
DAM
other names :
DNA adenine methyltransferase; DNA adenine methylase; DNA adenine methyltransferase; DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.EcoDam
other gene names :
dam; dam; ECK3374; JW3350
uniprot entry name :
DMA_ECOLI
clonality :
Monoclonal
isotype :
IgG1
clone :
RB1
host :
Mouse
reactivity :
Gram Negative Bacteria
sequence length :
278
purity :
Protein A/G Chromatography
form :
Unconjugated
concentration :
1mg/ml
storage stability :
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles. Shipping: Ship at ambient temperature, freeze upon arrival. Stability: Products are stable for one year from purchase when stored properly
tested application :
Western Blot (WB)
app notes :
Antibody can be used for Western Blotting (1-5ug/ml). Other applications not yet evaluated. Optimal concentration should be evaluated by serial dilutions.
other info1 :
Immunogen: Recombinant DNA Adenine Methylase protein.
other info2 :
Positive Control: Not available. Acession Number: P0AEE8, E.coli
products categories :
Monoclonal Antibody
products description :
DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.
ncbi gi num :
16131265
ncbi acc num :
NP_417846.1
ncbi gb acc num :
NC_000913.2
uniprot acc num :
P0AEE8
ncbi pathways :
Mismatch Repair Pathway (1128); Mismatch Repair Pathway (453)
ncbi summary :
Dam is a base flipping enzyme. [More information is available at EcoGene: EG10204]. DNA adenine methyltransferase (Dam), which is responsible for methylation of nearly all GATC sequences in Escherichia coli , is encoded by the dam gene and occurs at about 130 molecules per rapidly growing cell . [More information is available at EcoCyc: EG10204].
uniprot summary :
Function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication. Catalytic activity: S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine. Sequence similarities: Belongs to the N(4)/N(6)-methyltransferase family.
size1 :
0.1 mg
price1 :
315 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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