antibody

p53 (a.a. 371-380)

MBS395175

0.1 mg

315 USD

monoclonal

mouse

nonconjugated

Pab122

human, mouse, rat

immunocytochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

p53 (a.a. 371-380)

p53 (a.a. 371-380)

apoptosis-inducing factor 2 isoform 2; Apoptosis-inducing factor 2; apoptosis-inducing factor 2; apoptosis-inducing factor-like mitchondrion-associated inducer of death; apoptosis-inducing factor-like mitochondrion-associated inducer of death; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; apoptosis-inducing factor homologous mitochondrion-associated inducer of death; apoptosis-inducing factor, mitochondrion-associated 2; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death

p53

Aifm2; Aifm2; Amid; PRG3; 5430437E11Rik; D730001I10Rik; Amid

AIFM2_MOUSE

Monoclonal

IgG2b

Pab122

Mouse

Human, Mouse, Rat, Monkey, Hamster

373

Protein A/G Chromatography

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles. Shipping: Ship at ambient temperature, freeze upton arrival. Stability: Products are stable for one year from puchase when stored properly.

Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Immunogen: Hybridoma produced by the fusion of splenocytes from immunized with full length mouse p53 protein and mouse myeloma cells.

Positive Control: Breast or colon carcinoma. Comment: THis antibody can be used for Western Blotting (5-10ug/ml, immunohistochemistry on frozen and formalin/paraffain embedded tissues (1-2ug/ml) and immunoprecipitation (2ug/mg of protein lysate). Optimal concentration should be evaluated by serial dilutions.

Monoclonal Antibody; p53, and Related/Induced Proteins

p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.

85861162

NP_001034283.1

NM_001039194.3

Q8BUE4

40,635 Da

Function: Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death . By similarity. May contribute to genotoxin-induced growth arrest. Cofactor: FAD. Binds 6-hydroxy-FAD non-covalently . By similarity. Subcellular location: Cytoplasm. Mitochondrion outer membrane . By similarity. Tissue specificity: Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in liver, testis, and kidney, and expressed at lower levels in pancreas, spleen, brain and lung. Ref.4. Induction: Expression is up-regulated in mouse embryonic fibroblasts by genotoxic reagents 5-fluorouracil and etoposide. Ref.4. Sequence similarities: Belongs to the FAD-dependent oxidoreductase family. Sequence caution: Isoform 2: The sequence AAH38129.1 differs from that shown. Reason: erroneous termination (Translated as Trp in position: 339:Stop->W)

0.1 mg

315 USD