antibody

RFP Tag (Red Fluorescent Protein)

MBS395142

0.1 mg

255 USD

monoclonal

mouse

nonconjugated

RFD5R

western blot, ELISA, immunoprecipitation, enzyme immunoassay

Antibody

RFP Tag (Red Fluorescent Protein)

RFP Tag (Red Fluorescent Protein)

RFP; DsRed

Monoclonal

IgG1

RFD5R

Mouse

Recognizes native and denatured forms of RFP and its variants: tag- RFP, turbo-RFP, DeRed, mCherry,

Protein A/G Chromatography

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product should be stored at -20 degree C. Products are stable for one year from purchase when stored properly. Aliquot to avoid freeze/thaw cycles

Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS)

Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution. In order to obtain best results in other assays (e.g. Dot, ELISA, IP, etc.), optimal concentration should be evaluated by serial dilutions.

Immunogen: Synthetic peptide from RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

Positive Control: Recognizes nativeand denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, tdTomato, etc.

Monoclonal Antibody; Expression/Fusion Protein Tags

Red Fluorescent Protein is thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

25.931

0.1 mg

255 USD