product summary
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company name :
MyBioSource
product type :
antibody
product name :
GAL-4 Activation Domain
catalog :
MBS395014
quantity :
0.1 mg
price :
340 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[5C8-5]
reactivity :
Saccharomyces cerevisiae S288c
application :
western blot, immunoprecipitation
more info or order :
product information
catalog number :
MBS395014
products type :
Antibody
products full name :
GAL-4 Activation Domain
products short name :
[GAL-4 Activation Domain]
other names :
[Gal4p; Regulatory protein GAL4; Gal4p]
other gene names :
[GAL4; GAL4; GAL81]
uniprot entry name :
GAL4_YEAST
clonality :
Monoclonal
isotype :
IgG1
clone :
[5C8-5]
host :
Mouse
reactivity :
Yeast
sequence length :
881
purity :
Protein A/G Chromatography
form :
Unconjugated. Provided as solution in phosphate buffered saline with 0.08% sodium azide
storage stability :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles. Shipping conditions: Ship at ambient temperature, freeze upon arrival.
tested application :
Western Blot (WB), Immunoprecipitation (IP)
app notes :
Antibody can be used for Western Blotting (5-10 ug/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be eevaluated by serial dilutions.
other info1 :
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.
other info2 :
Functional Analysis: Western Blotting
products categories :
Monoclonal Antibody; Expression/Fusion Protein Tags
products description :
GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
ncbi gi num :
6325008
ncbi acc num :
NP_015076.1
ncbi gb acc num :
NM_001184062.1
uniprot acc num :
P04386
ncbi mol weight :
99,403 Da
uniprot summary :
Function: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Ref.5 Ref.6. Subcellular location: Nucleus. Domain: the 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.8. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. Miscellaneous: Present with 166 molecules/cell in log phase SD medium. Sequence similarities: Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.
size1 :
0.1 mg
price1 :
340 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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