catalog number :
MBS378270
products type :
ELISA Kit
products full name :
Pembrolizumab(Keytruda) Pharmacokinetic
products short name :
[Pembrolizumab(Keytruda) Pharmacokinetic]
specificity :
sTNFa, TNFb, sTNFRI, sTNFRII prepared at 250 ng/mL were assayed and exhibited no crossreactivity or interference.
storage stability :
Store kit components at -20 degree C unless specified otherwise
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Assay Type: Sandwich. Detection Range: 1.5ng/mL.
products description :
Principle of the Assay: This assay employs the sandwich enzyme immunoassay technique. Capture antibody is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Etanercept present in biological matrices is bound by the immobilized anti-Etanercept antibody. After washing away any unbound substances, enzyme linked anti-Etanercept antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Etanercept present in test samples. The color development is stopped and the intensity of the color is measured!!Background/Introduction: Etanercept (Enbrel) is a protein drug used to treat autoimmune diseases by adsorbing tumor necrosis factor (TNF; a soluble inflammatory cytokine). Etanercept is a fusion protein of the extracellular ligandbinding portion of TNFRSF1B and the Fc component of human immunoglobulin G1 (IgG1). It reduces the effect of naturally present TNF, functioning as a decoy receptor that binds to TNF. The Etanercept ELISA kit is designed to measure free Etanercept with high specificity and sensitivity in biological matrices.