catalog number :
MBS378018
products type :
ELISA Kit
products full name :
Omalizumab (Xolair) PK ELISA Kit
products short name :
[Omalizumab (Xolair) PK]
specificity :
hIgG1, Rituximab, and Infliximab prepared at 250 ng/mL were assayed and exhibited no cross-reactivity or interference.
storage stability :
Shelf life: 6 months from date of shipment.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Assay Type: Sandwich. Detection Range: 1.56 ng/mL.
products description :
Principle of the Assay: This assay employs the sandwich enzyme immunoassay technique. Anti-Cetuximab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Cetuximab present in biological matrices is bound by the immobilized anti-Cetuximab antibody. After washing away any unbound substances, enzyme linked anti-Cetuximab antibody is added to the wells. This antibody is developed and purified specifically against truncated Erbitux (domain residing in Fc portion of the Erbitux molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Cetuximab present in test samples. The color development is stopped and the intensity of the color is measured!!Background/Introduction: Cetuximab (Erbitux) is a chimeric IgG1 monoclonal antibody that binds the extra-cellular domain of the epidermal growth factor receptor (EGFR). Cetuximab inhibits activation of the EGFR and the associated downstream signaling. Furthermore Cetuximab induces antibody-mediated receptor dimerization, internalization and degradation leading to receptor down-regulation, and antibody-dependent cellular cytotoxicity that could contribute to its antitumor effect. The Cetuximab ELISA kit is designed to measure Cetuximab with high specificity and enhanced sensitivity in serum or plasma.