catalog number :
MBS378007
products type :
ELISA Kit
products full name :
Infliximab (Remicade) PK
products short name :
[Infliximab (Remicade) PK]
specificity :
hIgG1 and Infliximab prepared at 250 ng/ mL were assayed and exhibited no cross-reactivity or interference.
storage stability :
Shelf life: 6 months from date of shipment.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Assay Type: Sandwich. Detection Range: 0.78 ng/mL.
products description :
Principle of the Assay: This assay employs the sandwich enzyme immunoassay technique. Anti- Infliximab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Infliximab present in biological matrices is bound by the immobilized anti- Infliximab antibody. After washing away any unbound substances, enzyme linked anti- Infliximab antibody is added to the wells. This antibody is developed and purified specifically against truncated Remicade (domain residing in Fc portion of the Remicade molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Infliximab present in test samples. The color development is stopped and the intensity of the color is measured. Background: Infliximab (Remicade) is a chimeric monoclonal antibody against tumour necrosis factor alpha (TNF- alpha). Infliximab neutralizes the biological activity of TNF- alpha by binding with high affinity to the soluble (free floating in the blood) and transmembrane forms of TNF-alpha, and thereby blocks binding of TNF-alpha with its receptors. The Infliximab ELISA kit is designed to measure free Infliximab with high specificity and sensitivity in biological matrices.