ELISA/assay

Etanercept (Enbrel) PK

MBS378003

1 x96 Wells

625 USD

ELISA Kit

Etanercept (Enbrel) PK

Etanercept (Enbrel) PK

sTNFa, TNFb, sTNFRI, sTNFRII prepared at 250 ng/mL were assayed and exhibited no cross-reactivity or interference.

Shelf life: 6 months from date of shipment.

Samples: Serum and plasma. Assay Type: Sandwich. Detection Range: 78 ng/mL.

Principle of the Assay This assay employs the sandwich enzyme immunoassay technique. Capture antibody is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Etanercept present in biological matrices is bound by the immobilized anti-Etanercept antibody. After washing away any unbound substances, enzyme linked anti-Etanercept antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Etanercept present in test samples. The color development is stopped and the intensity of the color is measured. Background/Introduction: Etanercept (trade name Enbrel) is a protein drug used to treat autoimmune diseases by adsorbing tumor necrosis factor (TNF; a soluble inflammatory cytokine). Etanercept is a fusion protein produced through expression of recombinant DNA by linking the extracellular ligand-binding portion of TNFRSF1B to the Fc component of human immunoglobulin G1 (IgG1). It reduces the effect of naturally present TNF, functioning as a decoy receptor that binds to TNF. Etanercept is indicated for the treatment of moderate to severe rheumatoid arthritis (RA), psoriatic arthritis, ankylosing spondylitis, and moderately to severely active polyarticular juvenile idiopathic arthritis. Serum concentration of ENBREL may predict some clinical outcome during maintenance therapy. In clinical trials Etanercept has been reported to elicit an antibody response. Both binding and neutralizing antibodies against Etanercept have been reported. The formation of binding or neutralizing antibodies to therapeutic agent may decrease the efficacy of these agents leading to losses of clinical response over time. Anti-drug antibodies may cause infusion reactions and anaphylactic reactions. Detection, measurement, and characterization of anti-therapeutic antibody are critical in understanding the safety, exposure, and efficacy profile of the therapeutic. The Etanercept ELISA kit is designed to measure free Etanercept with high specificity and sensitivity. The assay design uses a pair of antibodies that allows detection of whole Etanercept molecules in biological matrices.

1 x96 Wells

625 USD