Mouse Anti-Lamin B1 [119D5-F1] - Nuclear Envelope Marker | MyBioSource MBS375244 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Lamin B1 [119D5-F1] - Nuclear Envelope Marker

catalog :

MBS375244

quantity :

0.05 mL

price :

370 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

119D5-F1

reactivity :

human, mouse, rat, dog, cow, zebrafish

application :

immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

product information

catalog number :

MBS375244

products type :

Antibody

products full name :

Mouse Anti-Lamin B1 [119D5-F1] - Nuclear Envelope Marker

products short name :

Lamin B1

products name syn :

Lamin B1 antibody; Lamin B2 antibody; LMN antibody; LMN B2 antibody; LMN2 antibody; LMNBantibody; LMNB1 antibody; LMNB2 antibody

other names :

lamin B1; Lamin-B1; lamin-B1; lamin B1

other gene names :

LMNB1; LMNB1; LMN; ADLD; LMN2; LMNB; LMN2; LMNB

uniprot entry name :

LMNB1_HUMAN

clonality :

Monoclonal

isotype :

IgG1 kappa

clone :

119D5-F1

host :

Mouse

reactivity :

Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Zebrafish

sequence length :

586

specificity :

Reacts with an epitope located C-terminal of residue 231 in lamin B1, does not cross react with lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. It has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested

form :

Purified antibody in PBS containing 0.09% sodium azide;

concentration :

1mg/ml

storage stability :

Store at 4 degree C for one month, or in small aliquots at -20 degree C for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

tested application :

ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immmunoblotting.

app notes :

Optimal antibody dilution should be determined by titration; recommended range is 1:100-1:200 for Flow Cytometry: 1/100 - 1/200, and for immunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100-1:1000 for immunoblotting applications.

other info1 :

Target Antigen: Lamin B1 - Nuclear Envelope Marker. Immunogen: Purified rat liver lamins

other info2 :

Myeloma/fusion Partners: P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse. Research Areas: Apoptosis; Cell Biology; Class V Lamins; Cytoskeleton; Cytoskeleton / ECM; Intermediate Filaments; Lamins; Nuclear Envelope; Nucleus; Subcellular Markers; Tags Cell Markers

products references :

Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes Weaver VM (J Cell Sci,Jan 1996) PUBMED Ehrnhoefer DE et al. A quantitative method for the specific assessment of caspase-6 activity in cell culture. PLoS One 6:e27680 (2011). WB ; Mouse PUBMED Ciciarello M et al. Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells. Chromosoma 119:651-68 (2010). ICC/IF ; Mouse PUBMED Kanninen K et al. Intrahippocampal injection of a lentiviral vector expressing Nrf2 improves spatial learning in a mouse model of Alzheimer's disease. Proc Natl Acad Sci U S A 106:16505-10 (2009) PUBMED Hui RC et al. The forkhead transcription factor FOXO3a increases phosphoinositide-3 kinase/Akt activity in drug-resistant leukemic cells through induction of PIK3CA expression. Mol Cell Biol 28:5886-98 (2008). WB ; Human PUBMED Thompson J & Winoto A During negative selection, Nur77 family proteins translocate to mitochondria where they associate with Bcl-2 and expose its proapoptotic BH3 domain. J Exp Med 205:1029-36 (2008). WB ; Mouse PUBMED Hui RC et al. Doxorubicin activates FOXO3a to induce the expression of multidrug resistance gene ABCB1 (MDR1) in K562 leukemic cells. Mol Cancer Ther 7:670-8 (2008). WB ; Human PUBMED Sharma S & Brosh RM Human RECQ1 is a DNA damage responsive protein required for genotoxic stress resistance and suppression of sister chromatid exchanges. PLoS ONE 2:e1297 (2007). WB ; Human PUBMED Ivorra C et al. A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C. Genes Dev 20:307-20 (2006) PUBMED Johnson MD et al. Proteome analysis of DNA damage-induced neuronal death using high throughput mass spectrometry. J Biol Chem 279:26685-97 (2004) PUBMED Machiels BM et al. Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults. J Pathol 182:197-204 (1997) PUBMED Krohne G. Lamins. Methods Cell Biol. 2004;78:573-96WB, ICC/IF, IP. Mouse, Human PMID: 15646632

ncbi gi num :

576840

ncbi acc num :

AAC37575.1

uniprot acc num :

P20700

ncbi mol weight :

66,408 Da

ncbi pathways :

Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Breakdown Of The Nuclear Lamina Pathway (105681); Caspase Cascade In Apoptosis Pathway (137974); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); DNA Damage/Telomere Stress Induced Senescence Pathway (905994)

ncbi summary :

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]

uniprot summary :

Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 5q23.2. Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope. Molecular Function: phospholipase binding; structural molecule activity. Biological Process: apoptosis; cell structure disassembly during apoptosis. Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant

size1 :

0.05 mL

price1 :

370 USD