antibody

Mouse Anti-Lamin A+C [JOL2]

MBS375085

1 mL

345 USD

monoclonal

mouse

nonconjugated

[JOL2]

western blot, immunoprecipitation

Antibody

Mouse Anti-Lamin A+C [JOL2]

[Lamin A+C]

[70 kDa Lamin antibody; Cardiomyopathy dilated 1A (autosomal dominant) antibody; CDCD1 antibody; CDDCantibody; Charcot Marie Tooth Disease Axonal Type 2B1 antibody; CMD1A antibody; CMT2B1 antibody; EMD2antibody; FPL antibody; FPLD antibody; HGPS antibody; IDC antibody; Lamin A/C antibody; Lamin A/C Isoform 1Precursor antibody; Lamin A/C Isoform 2 antibody; Lamin A/C Isoform 3 antibody; LDP1 antibody; LFPantibody; LGMD1B antibody; Limb girdle muscular dystrophy 1B (autosomal dominant) antibody; LMN 1 antibody; LMN Aantibody; LMN C antibody; LMN1 antibody; LMNA antibody; LMNC antibody; NY REN 32 antigen antibody; PRO1antibody; Progeria 1 (Hutchinson Gilford Type) antibody; Renal carcinoma antigen NY REN 32 antibody; Renalcarcinoma antigen NYREN32 antibody]

Monoclonal

IgG1

[JOL2]

Mouse

This antibody reacts with both recombinant and native Lamin A and C in humans. Binds to an epitope between amino acids 464-572

Unpurified

Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum, plus 0.09% sodium azide).

Store at 4°C for one month, or in small aliquots at -20°C for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

Immunoprecipitation , western blot, flow cytometry & immunohistology.

Optimal antibody dilution should be determined by titration, however as a guideline for western blot up to 1/200, and for immunoprecipitation use neat.

Immunohistochemistry (IHC)

Immunogen: Recombinant human lamin A

Myeloma/fusion Partners: Spleen cells from immunised BALB/C mice were fused with cells of the mouse). SP2-0/Ag4 myeloma cell line. Health & Safety: Products that contain sodium azide ( a poisonous and hazardous substance) should be handled by trained staff only.

Both emerin and lamin C depend on lamin A for localization at the nuclear envelope. Vaughan A (J Cell Sci,Jul 2001) PUBMED Iglesias-Guimarais V et al. Apoptotic DNA Degradation into Oligonucleosomal Fragments, but Not Apoptotic Nuclear Morphology, Relies on a Cytosolic Pool of DFF40/CAD Endonuclease. J Biol Chem 287:7766-79 (2012). WB ; Human PUBMED Harrigan JA et al. Replication stress induces 53BP1-containing OPT domains in G1 cells. J Cell Biol 193:97-108 (2011). ICC/IF ; Human PUBMED Gudise S et al. Samp1 is functionally associated with the LINC complex and A-type lamina networks. J Cell Sci 124:2077-85 (2011) PUBMED Harten IA et al. Age-Dependent Loss of MMP-3 in Hutchinson-Gilford Progeria Syndrome. J Gerontol A Biol Sci Med Sci : (2011) PUBMED Toulany M et al. Impact of oncogenic K-RAS on YB-1 phosphorylation induced by ionizing radiation. Breast Cancer Res 13:R28 (2011). WB ; Human PUBMED Mamchaoui K et al. Immortalized pathological human myoblasts: towards a universal tool for the study of neuromuscular disorders. Skelet Muscle 1:34 (2011). ICC/IF ; Human PUBMED Negroni E et al. In vivo myogenic potential of human CD133+ muscle-derived stem cells: a quantitative study. Mol Ther 17:1771-8 (2009). ICC/IF ; Human PUBMED

1 mL

345 USD

0.1 mL

410